Bennacef Chanez, Desobry Stéphane, Jasniewski Jordane, Leclerc Sébastien, Probst Laurent, Desobry-Banon Sylvie
Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), ENSAIA, 54000 Nancy, France.
Cookal Company, 19 Avenue de la Meurthe, 54320 Maxéville, France.
Polymers (Basel). 2023 Oct 20;15(20):4163. doi: 10.3390/polym15204163.
Two types of alginates, AlgLF and AlgP, were used in this study to produce alginate beads by electro-vibratory extrusion. AlgLF and AlgP exhibited different Mannuronate/Guluronate (M/G) ratios and molecular weights as measured by NMR and SEC-MALS. The calcium chloride concentration was found to have the greatest effect on bead size. Higher concentrations resulted in smaller beads. AlgLF with a higher molecular weight and a lower proportion of G blocks showed smaller beads. For both alginates, the bead size was also influenced by the flow rate and vibration frequency. Alginate solution aging showed a minimal effect. Alginate reticulation was modeled using a mathematical equation. The study provides insights for the optimization of alginate-based materials in different applications by shedding light on the main factors influencing bead size. The importance of the molecular weight, M/G ratio and calcium ion concentration in the gelling process is highlighted, providing opportunities for the tailoring of alginate materials through a phenomenological model.
本研究使用了两种藻酸盐AlgLF和AlgP，通过电动振动挤压法制备藻酸盐珠。通过核磁共振（NMR）和多角度激光光散射尺寸排阻色谱法（SEC-MALS）测定，AlgLF和AlgP表现出不同的甘露糖醛酸/古洛糖醛酸（M/G）比率和分子量。发现氯化钙浓度对珠粒尺寸影响最大。较高的浓度会导致较小的珠粒。具有较高分子量和较低比例G块的AlgLF显示出较小的珠粒。对于两种藻酸盐，珠粒尺寸还受流速和振动频率的影响。藻酸盐溶液老化的影响最小。使用数学方程对藻酸盐网状结构进行了建模。该研究通过揭示影响珠粒尺寸的主要因素，为不同应用中基于藻酸盐的材料的优化提供了见解。强调了分子量、M/G比率和钙离子浓度在凝胶化过程中的重要性，通过现象学模型为藻酸盐材料的定制提供了机会。
