MacGillivray R T, Irwin D M
Haemostasis. 1986;16(3-4):227-38. doi: 10.1159/000215295.
Using recombinant DNA techniques, DNA fragments coding for bovine prothrombin mRNA have been cloned and characterized. Structural studies have revealed that prothrombin mRNA encodes a precursor protein having an amino-terminal extension of 43 amino acid residues. Using bovine prothrombin cDNA as a hybridization probe, the genes coding for human and bovine prothrombin have been isolated and partially characterized. The organization of the prothrombin gene is similar, but not identical to the organization of the genes coding for the other vitamin-K-dependent clotting factors.
利用重组DNA技术，已对编码牛凝血酶原mRNA的DNA片段进行了克隆和表征。结构研究表明，凝血酶原mRNA编码一种前体蛋白，该前体蛋白具有一个由43个氨基酸残基组成的氨基末端延伸。以牛凝血酶原cDNA作为杂交探针，已分离出编码人和牛凝血酶原的基因并对其进行了部分表征。凝血酶原基因的组织方式与编码其他维生素K依赖性凝血因子的基因的组织方式相似，但并不完全相同。
