Cui Miao, Wang Zibin, Yang Yongchun, Liu Ru, Wu Min, Li Yujie, Zhang Qizhong, Xu Delin
College of Life Science and Technology, Jinan University, Guangzhou, China.
Front Genet. 2022 Mar 3;13:782957. doi: 10.3389/fgene.2022.782957. eCollection 2022.
Tilapia ( sp.) is one of the important economical fishes in the world. Streptococcosis is commonly found in tilapia, causing severe and devastating effects in tilapia cultures. and are the predominant pathogens causing tilapia streptococcosis. To understand the molecular mechanisms underlying differential streptococcal infection patterns, Nile tilapias () were infected by 1 × 10 CFU/mL , 1 × 10 CFU/mL , and 1 × 10 CFU/mL and (1:1), respectively, and transcriptome analysis was conducted to the intestine samples of Nile tilapia () at 6, 12, 24 h, and 7 days post-infection. A total of 6,185 genes that differentially expressed among groups were identified. Eight differentially expressed genes (DEGs) including , , , , , , , (), and , that involved in the immune system were selected, and their expression levels in the coinfection group were significantly higher than those in either of the single infection groups. These genes were associated with four different KEGG pathways. Additionally, the differential expression of eight DEGs was validated by using the RT-qPCR approach, and their immunological importance was discussed. The results provided insights into the responses of tilapia against and at the transcriptome level, promoting our better understanding of immune responses for aquatic animal against .
罗非鱼（罗非鱼属）是世界上重要的经济鱼类之一。链球菌病在罗非鱼中普遍存在，对罗非鱼养殖造成严重破坏。[此处原文缺失两种病原体名称]是引起罗非鱼链球菌病的主要病原体。为了解不同链球菌感染模式背后的分子机制，尼罗罗非鱼（尼罗罗非鱼）分别用1×10⁶CFU/mL[第一种病原体名称]、1×10⁷CFU/mL[第二种病原体名称]以及1×10⁶CFU/mL[第一种病原体名称]和[第二种病原体名称]（1:1）进行感染，并在感染后6、12、24小时和7天对尼罗罗非鱼（尼罗罗非鱼）的肠道样本进行转录组分析。共鉴定出6185个在组间差异表达的基因。选择了8个参与免疫系统的差异表达基因（DEGs），包括[此处原文缺失8个基因的具体名称]，其在混合感染组中的表达水平显著高于单一感染组中的任何一组。这些基因与4条不同的KEGG途径相关。此外，通过RT-qPCR方法验证了8个DEGs的差异表达，并讨论了它们的免疫学重要性。结果为罗非鱼在转录组水平上对[两种病原体名称]的反应提供了见解，有助于我们更好地理解水生动物对[两种病原体名称]的免疫反应。
