Kobayashi Y
J Dairy Sci. 1978 May;61(5):592-5. doi: 10.3168/jds.S0022-0302(78)94414-4.
A simplified resazurin rennet test for diagnosis of bovine mastitis was devised. A tablet of resazurin and rennet was placed in a sterile test tube to which 10 ml of milk were added directly from a teat. The test tube was incubated for 1 h at 37 C. After incubation the change in color of resazurin and the time required for coagulation by the rennet were observed. From the results the milk samples tested were classified into four types. Cell count, modified California Mastitis Test, coagulase positive staphylococci, and contents of solids-not-fat, lactose, and protein differed among the four types. This method is simple and practically useful for diagnosis of bovine mastitis.
设计了一种用于诊断牛乳腺炎的简化刃天青凝乳酶试验。将一片刃天青和凝乳酶置于无菌试管中，直接从乳头加入10毫升牛奶。将试管在37℃下孵育1小时。孵育后，观察刃天青的颜色变化以及凝乳酶凝结所需的时间。根据结果将测试的牛奶样本分为四类。四类样本的细胞计数、改良加利福尼亚乳腺炎试验、凝固酶阳性葡萄球菌以及非脂固形物、乳糖和蛋白质含量有所不同。该方法简单，对牛乳腺炎的诊断具有实际应用价值。
