Bonilla Hector, Hampton Dylan, Marques de Menezes Erika G, Deng Xutao, Montoya José G, Anderson Jill, Norris Philip J
Department of Medicine, Stanford University, Palo Alto, CA, United States.
Vitalant Research Institute, San Francisco, CA, United States.
Front Immunol. 2022 Mar 4;13:841910. doi: 10.3389/fimmu.2022.841910. eCollection 2022.
Myalgic encephalomyelitis, or chronic fatigue syndrome (ME/CFS) is a serious disease whose cause has yet to be identified. Objective markers of the disease are also not well understood and would serve as important tools in diagnosis and management. One potential biomarker or transmitter of immune signals in ME/CFS is the extracellular vesicle (EV) compartment. These small, membrane bound particles have been shown to play a key role in intercellular signaling. Our laboratory has focused on methods of detection of EVS in clinical samples. In this study we explored whether the prevalence of EVs in the plasma of participants with mild or severe ME/CFS differed from the plasma of healthy control participants. By staining for multiple cell surface molecules, plasma EVs could be fingerprinted as to their cell of origin. Our study revealed a significant correlation between severe ME/CSF and levels of EVs bearing the B cell marker CD19 and the platelet marker CD41a, though these changes were not significant after correction for multiple comparisons. These findings point to potential dysregulation of B cell and platelet activation or homeostasis in ME/CFS, which warrants validation in a replication cohort and further exploration of potential mechanisms underlying the association.
肌痛性脑脊髓炎，即慢性疲劳综合征（ME/CFS），是一种病因尚未明确的严重疾病。该疾病的客观标志物也尚未被充分了解，而它们将成为诊断和管理中的重要工具。ME/CFS中一种潜在的生物标志物或免疫信号传递者是细胞外囊泡（EV）区室。这些小的、有膜包裹的颗粒已被证明在细胞间信号传导中起关键作用。我们实验室专注于临床样本中细胞外囊泡的检测方法。在本研究中，我们探讨了轻度或重度ME/CFS参与者血浆中细胞外囊泡的患病率是否与健康对照参与者的血浆不同。通过对多种细胞表面分子进行染色，血浆细胞外囊泡可以根据其来源细胞进行指纹识别。我们的研究揭示了重度ME/CSF与携带B细胞标志物CD19和血小板标志物CD41a的细胞外囊泡水平之间存在显著相关性，尽管在进行多重比较校正后这些变化并不显著。这些发现表明ME/CFS中B细胞和血小板激活或稳态可能存在失调，这需要在重复队列中进行验证，并进一步探索该关联背后的潜在机制。
