Rex Devasahayam Arokia Balaya, Patil Arun H, Modi Prashant Kumar, Kandiyil Mrudula Kinarulla, Kasaragod Sandeep, Pinto Sneha M, Tanneru Nandita, Sijwali Puran Singh, Prasad Thottethodi Subrahmanya Keshava
Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore 575018, India.
CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500007, Telangana, India.
ACS Omega. 2022 Mar 2;7(10):8246-8257. doi: 10.1021/acsomega.1c03892. eCollection 2022 Mar 15.
Malaria is a vector-borne disease. It is caused by parasites. is a rodent model parasite, primarily used for studying parasite development in liver cells and vectors. To better understand parasite biology, we carried out a high-throughput-based proteomic analysis of . From the same mass spectrometry (MS)/MS data set, we also captured several post-translational modified peptides by following a bioinformatics analysis without any prior enrichment. Further, we carried out a proteogenomic analysis, which resulted in improvements to some of the existing gene models along with the identification of several novel genes. Analysis of proteome and post-translational modifications (PTMs) together resulted in the identification of 3124 proteins. The identified PTMs were found to be enriched in mitochondrial metabolic pathways. Subsequent bioinformatics analysis provided an insight into proteins associated with metabolic regulatory mechanisms. Among these, the tricarboxylic acid (TCA) cycle and the isoprenoid synthesis pathway are found to be essential for parasite survival and drug resistance. The proteogenomic analysis discovered 43 novel protein-coding genes. The availability of an in-depth proteomic landscape of a malaria pathogen model will likely facilitate further molecular-level investigations on pre-erythrocytic stages of malaria.
疟疾是一种媒介传播疾病。它由寄生虫引起。[具体寄生虫名称未给出]是一种啮齿动物模型寄生虫，主要用于研究寄生虫在肝细胞和媒介中的发育。为了更好地理解寄生虫生物学，我们对[具体寄生虫名称未给出]进行了基于高通量的蛋白质组学分析。从同一质谱（MS）/MS数据集，我们还通过生物信息学分析捕获了几种翻译后修饰肽，无需任何预先富集。此外，我们进行了蛋白质基因组分析，这导致对一些现有基因模型的改进以及几个新基因的鉴定。蛋白质组和翻译后修饰（PTM）的分析共同鉴定出3124种蛋白质。发现鉴定出的PTM在线粒体代谢途径中富集。随后的生物信息学分析深入了解了与代谢调节机制相关的蛋白质。其中，三羧酸（TCA）循环和类异戊二烯合成途径对于寄生虫的存活和耐药性至关重要。蛋白质基因组分析发现了43个新蛋白质编码基因。疟疾病原体模型深入蛋白质组图谱的可用性可能会促进对疟疾红细胞前期阶段的进一步分子水平研究。
