Jiang Xiulin, Shi Yulin, Chen Xi, Xu Haitao, Huang Xiaobin, Li Lihua, Pu Jun
Department of Neurosurgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, China.
Front Mol Biosci. 2022 Mar 3;9:773662. doi: 10.3389/fmolb.2022.773662. eCollection 2022.
Gliomas account for 75% of all primary malignant brain tumors in adults and are associated with high mortality. Emerging evidence has demonstrated that baculoviral inhibitor of apoptosis repeat containing 5 () plays a critical role in cell apoptosis and the progression of diverse cancers. However, no studies have yet focused on the immunological function and mechanisms of upstream regulation in the progression of low-grade gliomas (LGG). Here, we evaluated expression and clinical characteristics in people with LGG using the Chinese Glioma Genome Atlas, The Cancer Genome Atlas, Gene Expression Omnibus, Rembrandt, and Gravendeel databases. We used Kaplan-Meier statistics and receiver operating characteristic (ROC) curves to analyze the prognostic value of in LGG. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment terms were also explored to identify functional roles of . The Tumor Immune Estimation Resource (TIMER) and Tumor Immune System Interaction (TISIDB) databases were used to examine the correlation between expression and immune cell infiltration in LGG. The Genomics of Drug Sensitivity in Cancer (GDSC) and Cancer Therapeutics Response Portal (CTRP) databases were used to examine the potential drugs targeting . We used transwell and wound healing assays to determine the biological functions of in glioma cell migration. Our results demonstrated that was highly expressed in LGG and the expression level correlated with tumor grade, prognosis, histological subtype, isocitrate dehydrogenase 1 () mutation, 1p/19q chromosomal co-deletion, chemotherapy status, and O[6]-methylguanine-DNA methyltransferase (MGMT) promoter methylation status. GO and KEGG analysis showed that is primarily involved in cell proliferation and immune response-related signaling pathways. We also found that was significantly correlated with m6A modification and diverse drug sensitivity. TIMER and TISIDB database analysis showed that expression is associated with infiltration of diverse immune cells and immune modulation in LGG. knockdown inhibited LGG cell migration. Collectively, our results demonstrate that is correlated with cell migration and immune infiltration in LGG and may be a useful prognostic biomarker.
胶质瘤占成人所有原发性恶性脑肿瘤的75%，且死亡率高。新出现的证据表明，含杆状病毒凋亡重复序列5（）在细胞凋亡和多种癌症的进展中起关键作用。然而，尚未有研究聚焦于低级别胶质瘤（LGG）进展过程中的免疫功能及上游调控机制。在此，我们使用中国胶质瘤基因组图谱、癌症基因组图谱、基因表达综合数据库、伦勃朗数据库和格拉文德尔数据库评估了LGG患者中的表达及临床特征。我们使用Kaplan-Meier统计法和受试者工作特征（ROC）曲线分析了在LGG中的预后价值。还探索了京都基因与基因组百科全书（KEGG）和基因本体论（GO）富集术语，以确定的功能作用。使用肿瘤免疫估计资源（TIMER）和肿瘤免疫系统相互作用（TISIDB）数据库来研究LGG中表达与免疫细胞浸润之间的相关性。使用癌症药物敏感性基因组学（GDSC）和癌症治疗反应门户（CTRP）数据库来研究靶向的潜在药物。我们使用Transwell和伤口愈合试验来确定在胶质瘤细胞迁移中的生物学功能。我们的结果表明，在LGG中高表达，且表达水平与肿瘤分级、预后、组织学亚型、异柠檬酸脱氢酶1（）突变、1p/19q染色体共缺失、化疗状态和O[6]-甲基鸟嘌呤-DNA甲基转移酶（MGMT）启动子甲基化状态相关。GO和KEGG分析表明，主要参与细胞增殖和免疫反应相关信号通路。我们还发现与m6A修饰和多种药物敏感性显著相关。TIMER和TISIDB数据库分析表明，LGG中的表达与多种免疫细胞浸润和免疫调节相关。敲低可抑制LGG细胞迁移。总的来说，我们的结果表明，与LGG中的细胞迁移和免疫浸润相关，可能是一个有用的预后生物标志物。
