Kesarwani Vasudha, Singh N P, Kashyap Bineeta, Kumar Amit
Department of Microbiology, Super Speciality Cancer Institute and Hospital, Lucknow, Uttar Pradesh, India.
Department of Microbiology, UCMS and GTB Hospital, Delhi, India.
J Family Med Prim Care. 2022 Jan;11(1):97-101. doi: 10.4103/jfmpc.jfmpc_584_21. Epub 2022 Jan 31.
To detect on stool specimens by polymerase chain reaction (PCR) among patients with pulmonary tuberculosis. Detection of complex in sputum forms the basis of diagnosis of pulmonary tuberculosis. However, some patients tend to swallow sputum and some are unable to produce sputum. Based on the survival of in the gastric fluid, swallowed organisms may be detectable in stool samples.
The study was carried out on 30 cases each in four groups: sputum smear-positive and sputum smear-negative adults, pediatric patients suspected of pulmonary tuberculosis along with healthy controls. The samples were processed for direct microscopy for acid-fast bacilli (AFB) and culture. Stool PCR was done on all 120 samples.
AFB was demonstrated in 42 and cultured in 39 out of 240 samples. PCR-targeting IS6110 gene showed positive results in 24 (20%) out of 120 stool samples. PCR in stool showed the highest positivity in sputum smear-positive samples followed by gastric aspirates and sputum smear-negative samples.
Stool PCR is a potentially useful diagnostic method for pulmonary tuberculosis.
通过聚合酶链反应（PCR）检测肺结核患者粪便标本中的[具体物质未明确]。检测痰液中的[具体物质未明确]复合物是肺结核诊断的基础。然而，一些患者倾向于吞咽痰液，还有一些患者无法咳痰。基于[具体物质未明确]在胃液中的存活情况，吞咽的病原体可能在粪便样本中被检测到。
对四组患者进行研究，每组30例，分别为痰涂片阳性和痰涂片阴性的成年人、疑似肺结核的儿科患者以及健康对照。对样本进行抗酸杆菌（AFB）直接显微镜检查和[具体物质未明确]培养。对所有120份样本进行粪便PCR检测。
240份样本中，42份样本检测到抗酸杆菌，39份样本培养出[具体物质未明确]。针对IS6110基因的粪便PCR检测在120份粪便样本中有24份（20%）呈阳性结果。粪便PCR检测在痰涂片阳性样本中的阳性率最高，其次是胃液抽吸物和痰涂片阴性样本。
粪便PCR是一种对肺结核潜在有用的诊断方法。
