Franco Dalenogare Jéssica, de Souza Vencato Marina, Franciele Feyh Dos Santos Montagner Greice, Duarte Thiago, Maria Medeiros Frescura Duarte Marta, Camponogara Camila, Marchesan Oliveira Sara, Leite da Veiga Marcelo, de Ugalde Marques da Rocha Maria Izabel, Pavanato Maria Amália, de Freitas Bauermann Liliane
Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, Brazil.
Department of Morphology, Federal University of Santa Maria, Santa Maria, Brazil.
Evid Based Complement Alternat Med. 2022 Mar 10;2022:7562569. doi: 10.1155/2022/7562569. eCollection 2022.
Cubiu, an Amazonian fruit, is widely used as food and popular treatment for pathologies that present an inflammatory pattern, such as skin wound healing. However, there is still no confirmation in the scientific literature about the safety profile, as well as the anti-inflammatory, antioxidant, and healing actions of cubiu. This study is divided into two experimental protocols using Wistar rats. Thus, the first objective (protocol 1) of this study was to evaluate the toxicity of an oral administration of cubiu extract at different doses for 28 days. The macroscopic and microscopic analyses of the liver and kidney were performed, and the following analysis was determined in plasma: glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, gamma-glutamyl transpeptidase, glucose, triglycerides, total cholesterol, urea, creatinine, and uric acid. After, we conducted the second protocol aimed to establish the potential antioxidant and anti-inflammatory capacity of cubiu and its interaction with magnetic field in skin wound healing. On days 3, 7, and 14 of treatment, skin and blood samples were collected and analyzed: the oxidative stress biomarkers (reactive substances to thiobarbituric acid, nonprotein thiols, superoxide dismutase, catalase, and glutathione S-transferase), myeloperoxidase enzymatic activity, and cytokines levels (interleukin 1, interleukin 6, interleukin 10, and tumor necrosis factor-alpha). The cubiu has shown to be safe and nontoxic. Both cubiu and magnetic field promoted decreased levels of proinflammatory and prooxidant biomarkers (interleukin 1, interleukin 6, tumor necrosis factor-alpha, and reactive substances to thiobarbituric acid), as well as increased levels of anti-inflammatory and antioxidant biomarkers (interleukin 10, nonprotein thiols, and superoxide dismutase), with greater potential when treatments are used in association. Thus, cubiu promotes antioxidant and anti-inflammatory action in skin wound healing, while also improving results of the conventional treatment for skin healing (magnetic field) when used in association.
库比乌是一种亚马逊水果，被广泛用作食物，并常用于治疗呈现炎症模式的病症，如皮肤伤口愈合。然而，科学文献中仍未证实库比乌的安全性概况以及其抗炎、抗氧化和愈合作用。本研究使用Wistar大鼠分为两个实验方案。因此，本研究的第一个目标（方案1）是评估连续28天口服不同剂量库比乌提取物的毒性。对肝脏和肾脏进行了宏观和微观分析，并测定了血浆中的以下指标：谷草转氨酶、谷丙转氨酶、γ-谷氨酰转肽酶、葡萄糖、甘油三酯、总胆固醇、尿素、肌酐和尿酸。之后，我们进行了第二个方案，旨在确定库比乌在皮肤伤口愈合中的潜在抗氧化和抗炎能力及其与磁场的相互作用。在治疗的第3天、第7天和第14天，采集皮肤和血液样本并进行分析：氧化应激生物标志物（硫代巴比妥酸反应性物质、非蛋白硫醇、超氧化物歧化酶、过氧化氢酶和谷胱甘肽S-转移酶）、髓过氧化物酶活性和细胞因子水平（白细胞介素1、白细胞介素6、白细胞介素10和肿瘤坏死因子-α）。结果表明库比乌是安全无毒的。库比乌和磁场均能降低促炎和促氧化生物标志物（白细胞介素1、白细胞介素6、肿瘤坏死因子-α和硫代巴比妥酸反应性物质）的水平，同时提高抗炎和抗氧化生物标志物（白细胞介素10、非蛋白硫醇和超氧化物歧化酶）的水平，联合使用时潜力更大。因此，库比乌在皮肤伤口愈合中具有抗氧化和抗炎作用，同时与传统皮肤愈合治疗方法（磁场）联合使用时还能改善治疗效果。
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