Kant Vinay, Gopal Anu, Pathak Nitya N, Kumar Pawan, Tandan Surendra K, Kumar Dinesh
Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, 243 122 UP, India.
Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, 243 122 UP, India.
Int Immunopharmacol. 2014 Jun;20(2):322-30. doi: 10.1016/j.intimp.2014.03.009. Epub 2014 Mar 24.
Prolonged inflammation and increased oxidative stress impairs healing in diabetics and application of curcumin, a well known antioxidant and anti-inflammatory agent, could be an important strategy in improving impaired healing in diabetics. So, the present study was conducted to evaluate the cutaneous wound healing potential of topically applied curcumin in diabetic rats. Open excision skin wound was created in streptozotocin induced diabetic rats and wounded rats were divided into three groups; i) control, ii) gel-treated and iii) curcumin-treated. Pluronic F-127 gel (25%) and curcumin (0.3%) in pluronic gel were topically applied in the gel- and curcumin-treated groups, respectively, once daily for 19 days. Curcumin application increased the wound contraction and decreased the expressions of inflammatory cytokines/enzymes i.e. tumor necrosis factor-alpha, interleukin (IL)-1beta and matrix metalloproteinase-9. Curcumin also increased the levels of anti-inflammatory cytokine i.e. IL-10 and antioxidant enzymes i.e. superoxide dismutase, catalase and glutathione peroxidase. Histopathologically, the curcumin-treated wounds showed better granulation tissue dominated by marked fibroblast proliferation and collagen deposition, and wounds were covered by thick regenerated epithelial layer. These findings reveal that the anti-inflammatory and antioxidant potential of curcumin caused faster and better wound healing in diabetic rats and curcumin could be an additional novel therapeutic agent in the management of impaired wound healing in diabetics.
长期炎症和氧化应激增加会损害糖尿病患者的伤口愈合，而姜黄素作为一种著名的抗氧化剂和抗炎剂，其应用可能是改善糖尿病患者伤口愈合受损的重要策略。因此，本研究旨在评估局部应用姜黄素对糖尿病大鼠皮肤伤口的愈合潜力。在链脲佐菌素诱导的糖尿病大鼠身上制造开放性切除皮肤伤口，将受伤大鼠分为三组：i）对照组，ii）凝胶治疗组，iii）姜黄素治疗组。分别在凝胶治疗组和姜黄素治疗组中局部应用普朗尼克F - 127凝胶（25%）和普朗尼克凝胶中的姜黄素（0.3%），每天一次，持续19天。应用姜黄素可增加伤口收缩，并降低炎症细胞因子/酶即肿瘤坏死因子-α、白细胞介素（IL）- 1β和基质金属蛋白酶-9的表达。姜黄素还增加了抗炎细胞因子即IL - 10的水平以及抗氧化酶即超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的水平。组织病理学上，姜黄素治疗的伤口显示出更好的肉芽组织，以明显的成纤维细胞增殖和胶原沉积为主，伤口被厚厚的再生上皮层覆盖。这些发现表明，姜黄素的抗炎和抗氧化潜力可使糖尿病大鼠伤口愈合更快、更好，姜黄素可能是治疗糖尿病患者伤口愈合受损问题的一种新的治疗药物。
