Liu Ruiqi, Hu Huan, McNeil Melissa, Xu Jiuzhi, Bi Xueyun, Lou Pengbo, Guerrero-Juarez Christian F, Dai Xing, Plikus Maksim V, Shuai Jianwei, Yu Zhengquan, Lv Cong
State Key Laboratories for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Department of Physics, and National Institute for Data Science in Health and Medicine, Xiamen University, Xiamen 361005, China.
iScience. 2022 Feb 26;25(3):103982. doi: 10.1016/j.isci.2022.103982. eCollection 2022 Mar 18.
The Mammary gland undergoes complicated epithelial remodeling to form lobuloalveoli during pregnancy, in which basal epithelial cells remarkably increase to form a basket-like architecture. However, it remains largely unknown how dormant mammary basal stem/progenitor cells involve in lobuloalveolar development. Here, we show that expression marks a rare population of mammary epithelial cells with the majority being basal epithelial cells. reporter-marked basal epithelial cells are relatively dormant mammary stem/progenitor cells. Although reporter-marked basal epithelial cells have limited contribution to the homeostasis of mammary epithelium, they divide rapidly during pregnancy and contribute to lobuloalveolar development. Furthermore, reporter-marked basal epithelial cells are preferentially used for multiple pregnancies. Using single-cell RNA-seq analysis, we identify multiple functionally distinct clusters within the reporter-marked cell-derived progeny cells during pregnancy. Taken together, our findings underscore reporter-marked basal cells as dormant stem/progenitor cells that contribute to mammary lobuloalveolar development during pregnancy.
乳腺在怀孕期间经历复杂的上皮重塑以形成小叶腺泡，在此过程中基底上皮细胞显著增加，形成篮状结构。然而，乳腺基底干/祖细胞如何参与小叶腺泡发育在很大程度上仍不清楚。在这里，我们表明，[具体基因名称]表达标记了一群罕见的乳腺上皮细胞，其中大多数是基底上皮细胞。[具体基因名称]报告基因标记的基底上皮细胞是相对静止的乳腺干/祖细胞。虽然[具体基因名称]报告基因标记的基底上皮细胞对乳腺上皮的稳态贡献有限，但它们在怀孕期间迅速分裂并促进小叶腺泡发育。此外，[具体基因名称]报告基因标记的基底上皮细胞优先用于多次妊娠。通过单细胞RNA测序分析，我们在怀孕期间[具体基因名称]报告基因标记的细胞衍生后代细胞中鉴定出多个功能不同的簇。综上所述，我们的研究结果强调了[具体基因名称]报告基因标记的基底细胞作为静止的干/祖细胞，在怀孕期间对乳腺小叶腺泡发育有贡献。
