Department of Geriatric (Urology), Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei, China.
Department of Urology, Wuhan Hankou Hospital, Wuhan, Hubei, China.
Cell Cycle. 2022 Jul;21(14):1479-1490. doi: 10.1080/15384101.2022.2054095. Epub 2022 Mar 20.
Bladder cancer is one of the most severe life-threatening illnesses worldwide. To contribute to a solution to this public health issue, here, we sought to identify a novel biomarker for the early diagnosis of bladder tumors. We conducted RNA sequence analysis utilizing samples from tumorous tissue and adjacent healthy tissue in bladder cancer patients and found that was upregulated in bladder tumor tissues, suggesting that it might be a candidate for involvement in bladder tumorigenesis. Accordingly, we performed a series of experiments to further verify the role of in bladder tumor progression. Our results revealed that promoted bladder tumor cell viability, proliferation, and adhesion, while diminishing bladder tumor cell apoptosis. We also focused on the role of epigenetics in bladder tumors and verified that was a miR-31-5p target gene, and its positive effect on bladder tumor progression was relieved by miR-31-5p. Overall, this study sheds new light regarding a novel oncogenic regulatory axis, /miR-31-5p, which is related to bladder tumor growth.
膀胱癌是全球最严重的威胁生命的疾病之一。为了为这一公共健康问题的解决方案做出贡献，我们在这里试图确定一种新的生物标志物，用于膀胱癌的早期诊断。我们利用膀胱癌患者的肿瘤组织和相邻健康组织的样本进行了 RNA 序列分析，发现 在膀胱癌组织中上调，表明它可能是参与膀胱癌发生的候选物。因此，我们进行了一系列实验来进一步验证 在膀胱肿瘤进展中的作用。我们的结果表明， 促进膀胱癌细胞的活力、增殖和黏附，同时减少膀胱肿瘤细胞的凋亡。我们还关注了表观遗传学在膀胱癌中的作用，并验证了 是 miR-31-5p 的靶基因，miR-31-5p 减轻了它对膀胱肿瘤进展的正向作用。总的来说，这项研究揭示了一个新的致癌调控轴，/miR-31-5p，与膀胱肿瘤的生长有关。
