血浆长链非编码 RNA MALAT1 的表达与绝经后骨质疏松症的疾病严重程度呈负相关。

Plasma LncRNA MALAT1 Expressions Are Negatively Associated with Disease Severity of Postmenopausal Osteoporosis.

机构信息

Department of Endocrinology, The Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Wuxi, China.

出版信息

Lab Med. 2022 Sep 1;53(5):446-452. doi: 10.1093/labmed/lmac009.

DOI:10.1093/labmed/lmac009

PMID:35311990

Abstract

BACKGROUND

Long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 (LncRNA MALAT1) has been proven to promote osteogenesis in different health conditions. However, the role of plasma MALAT1 in postmenopausal osteoporosis (PMOP) has not been investigated.

OBJECTIVE

To investigate whether plasma MALAT1 expressions are associated with severity of PMOP.

METHODS

A total of 126 patients with PMOP and 126 healthy female control individuals were drafted into study participation. Plasma MALAT1 was detected using RT-PCR. Bone formation marker bone-specific alkaline phosphatase plasma concentration was determined using chemiluminescence immunoassay. Levels of bone absorption marker cross-linked N-telopeptidases of type I collagen were measured in duplicate using enzyme immunoassay. Bone mineral density (BMD) was examined in the total hips, femoral neck, and lumbar (L1-L4) spine using dual-energy x-ray absorptiometry. We used Genant semiquantitative (GSQ) criteria to assess the degree of vertebral deformity and fracture. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the potential diagnostic value of MALAT1 with regard to the GSQ grading. We used the Visual Analog Scale (VAS) and Oswestry Disability Index (ODI) to evaluate the symptomatic severity in and functional ability of the study participants.

RESULTS

Plasma MALAT1 expressions were significantly lower in patients with PMOP, compared with healthy controls. Plasma MALAT1 expressions in patients with PMOP were positively associated with total hip, femoral neck, and lumbar (L1-L4) spine BMD. In total, 95 patients experienced vertebral deformity or fracture (VF), and 31 had no fractures. Plasma MALAT1 expressions were markedly decreased in patients with VF, compared with patients without fractures. Plasma MALAT1 expressions were negatively related to GSQ grading in patients with VF. ROC curve analysis demonstrated that decreased plasma MALAT1 expression exhibits decent diagnostic value with regard to GSQ grading. Finally, we discovered that plasma MALAT1 expression was also negatively associated with VAS and ODI.

CONCLUSION

Plasma MALAT1 expressions are negatively associated with severity of PMOP.

摘要

背景

长链非编码 RNA 转移相关肺腺癌转录本 1（LncRNA MALAT1）已被证明在不同健康状况下促进成骨作用。然而，血浆 MALAT1 在绝经后骨质疏松症（PMOP）中的作用尚未被研究。

目的

探讨血浆 MALAT1 表达是否与 PMOP 的严重程度相关。

方法

共纳入 126 例 PMOP 患者和 126 例健康女性对照者参与研究。采用 RT-PCR 检测血浆 MALAT1。采用化学发光免疫法检测骨形成标志物骨碱性磷酸酶血浆浓度。采用酶联免疫吸附法重复检测骨吸收标志物Ⅰ型胶原交联 N-末端肽水平。采用双能 X 线吸收法检测全髋、股骨颈和腰椎（L1-L4）的骨密度（BMD）。采用 Genant 半定量（GSQ）标准评估椎体变形和骨折程度。采用受试者工作特征（ROC）曲线分析 MALAT1 对 GSQ 分级的潜在诊断价值。采用视觉模拟评分（VAS）和 Oswestry 功能障碍指数（ODI）评估研究参与者的症状严重程度和功能能力。

结果

与健康对照组相比，PMOP 患者的血浆 MALAT1 表达显著降低。PMOP 患者的血浆 MALAT1 表达与全髋、股骨颈和腰椎（L1-L4）BMD 呈正相关。共有 95 例患者发生椎体变形或骨折（VF），31 例患者无骨折。与无骨折患者相比，VF 患者的血浆 MALAT1 表达显著降低。VF 患者的血浆 MALAT1 表达与 GSQ 分级呈负相关。ROC 曲线分析表明，降低的血浆 MALAT1 表达对 GSQ 分级具有良好的诊断价值。最后，我们发现血浆 MALAT1 表达也与 VAS 和 ODI 呈负相关。