Nilsson Anders K, Rydbeck Halfdan, Thorsell Annika, Frändberg Sofia, Barreto Henriksson Helena, Hesse Camilla, Hellgren Gunnel, Lundgren Pia, Hellström Ann
The Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
The Sahlgrenska Centre for Pediatric Ophthalmology Research, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; The Bioinformatics Core Facility at the University of Gothenburg, Gothenburg, Sweden.
Stem Cell Res. 2022 May;61:102752. doi: 10.1016/j.scr.2022.102752. Epub 2022 Mar 14.
Hematopoietic stem and progenitor cells (HSPC) from umbilical cord blood (UCB) are used for transplantation to treat blood disorders. Methods to estimate the HSPC count in umbilical cord blood, and thereby identify high-value blood units, are time-consuming and costly. Recent studies indicate that the UCB plasma protein composition relates to the HSPC count. We compared the plasma proteome of UCB with high vs low HSPC cell count (>115 × 10 vs < 51 × 10 CD34 cells l) by using a combination of global untargeted MS quantitative proteomics and targeted proximity extension assay (PEA) proteomics. For the MS platform, 96 proteins differed significantly between the CD34 groups, and out of these, 44 proteins showed more than a two-fold difference. Seven pathways were enriched in high CD34 samples, including pathways relating to platelets, coagulation, and lipid transport. For the PEA platform, 61 proteins were differentially abundant, and among these 7 proteins showed more than a two-fold difference between groups. In the PEA data, a high CD34 cell count was associated with a protein hub with functions in platelet degranulation. We conclude that the HSPC count is related to the UCB plasma proteome, but that further studies are needed to discern if these findings reflect causal relationships.
来自脐带血（UCB）的造血干细胞和祖细胞（HSPC）被用于移植治疗血液疾病。估计脐带血中HSPC数量从而识别高价值血液单位的方法既耗时又昂贵。最近的研究表明，UCB血浆蛋白质组成与HSPC数量有关。我们通过结合全局非靶向质谱定量蛋白质组学和靶向邻近延伸分析（PEA）蛋白质组学，比较了HSPC细胞计数高（>115×10 vs <51×10 CD34细胞/升）与低的UCB血浆蛋白质组。对于质谱平台，CD34组之间有96种蛋白质存在显著差异，其中44种蛋白质的差异超过两倍。高CD34样本中有7条通路富集，包括与血小板、凝血和脂质转运相关的通路。对于PEA平台，有61种蛋白质丰度存在差异，其中7种蛋白质在组间差异超过两倍。在PEA数据中，高CD34细胞计数与在血小板脱颗粒中起作用的蛋白质枢纽相关。我们得出结论，HSPC数量与UCB血浆蛋白质组有关，但需要进一步研究以确定这些发现是否反映因果关系。
