Frändberg Sofia, Asp Julia, Waldner Berit, Holgersson Jan, Palmqvist Lars
Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden; Department of Clinical Immunology and Transfusion Medicine, Sahlgrenska University Hospital, Gothenburg, Sweden.
Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Sweden; Department of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden.
Stem Cell Res. 2018 May;29:24-27. doi: 10.1016/j.scr.2018.03.008. Epub 2018 Mar 16.
Successful hematopoietic stem and progenitor cell (HSPC) transplantation rests upon reliable methods for their enumeration in sources such as cord blood (CB). Methods used today are costly, time consuming and exhaust the limited number of cells needed for transplantation. The aim of this study was to analyze if surplus plasma from CB contains biomarkers that can predict HSPC content in CB. Frozen, surplus plasma from 95 CB units was divided into two groups based on CD34+ cell concentration. Birth weight, gestation age, gender, mode of delivery, collection volume, nucleated cell count and colony forming unit assay results were available. Samples were analyzed with a proximity ligation assay covering 92 different proteins. Two-group t-test with p-values adjusted for false discovery rate (FDR) identified 5 proteins that significantly differed between the two groups. CDCP1 was the most significant (FDR adjusted p-value 0.006). Correlation with CDCP1 concentration was most significant for CD34+ concentration and nucleated cell count. Multivariate analysis showed that CD34 and gender seemed to influence the level of CDCP1. In conclusion, CDCP1 was identified as a potential biomarker of HSPC content in CB. The finding also warrants further investigation for a possible role of CDCP1 in regulating HSPC presence in CB.
成功的造血干细胞和祖细胞（HSPC）移植依赖于在脐带血（CB）等来源中对其进行计数的可靠方法。目前使用的方法成本高、耗时，且会耗尽移植所需的有限数量的细胞。本研究的目的是分析CB中的多余血浆是否含有可预测CB中HSPC含量的生物标志物。根据CD34+细胞浓度，将来自95个CB单位的冷冻多余血浆分为两组。可获得出生体重、胎龄、性别、分娩方式、采集量、有核细胞计数和集落形成单位测定结果。用覆盖92种不同蛋白质的邻近连接分析法对样本进行分析。采用对错误发现率（FDR）进行校正的两组t检验，确定了两组之间有显著差异的5种蛋白质。CDCP1最为显著（FDR校正p值为0.006）。CDCP1浓度与CD34+浓度和有核细胞计数的相关性最为显著。多变量分析表明，CD34和性别似乎会影响CDCP1的水平。总之，CDCP1被确定为CB中HSPC含量的潜在生物标志物。这一发现也值得进一步研究CDCP1在调节CB中HSPC存在方面可能发挥的作用。
