Güven Göksel, Uz Zühre, Hilty Matthias P, Bilecenoğlu Burak, Akin Șakir, Ince Yasin, Ince Can
Department of Intensive Care, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
Department of Translational Physiology, Amsterdam University Medical Centers, Amsterdam, The Netherlands.
J Vasc Res. 2022;59(4):199-208. doi: 10.1159/000522394. Epub 2022 Mar 21.
Monitoring the sublingual and oral microcirculation (SM-OM) using hand-held vital microscopes (HVMs) has provided valuable insight into the (patho)physiology of diseases. However, the microvascular anatomy in a healthy population has not been adequately described yet.
Incident dark field-based HVM imaging was used to visualize the SM-OM. First, the SM was divided into four different fields; Field-a (between incisors-lingua), Field-b (between the canine-first premolar-lingua), Field-c (between the first-second premolar-lingua), Field-d (between the second molar-wisdom teeth-lingua). Second, we investigated the buccal area, lower and upper lip. Total/functional vessel density (TVD/FCD), focus depth (FD), small vessel mean diameters (SVMDs), and capillary tortuosity score (CTS) were compared between the areas.
Fifteen volunteers with a mean age of 29 ± 6 years were enrolled. No statistical difference was found between the sublingual fields in terms of TVD (p = 0.30), FCD (p = 0.38), and FD (p = 0.09). SVMD was similar in Field-a, Field-b, and Field-c (p = 0.20-0.30), and larger in Field-d (p < 0.01, p = 0.015). The CTS of the buccal area was higher than in the lips.
The sublingual area has a homogenous distribution in TVD, FCD, FD, and SVMD. This study can be a description of the normal microvascular anatomy for future researches regarding microcirculatory assessment.
使用手持式活体显微镜（HVM）监测舌下和口腔微循环（SM-OM）为疾病的（病理）生理学提供了有价值的见解。然而，健康人群的微血管解剖结构尚未得到充分描述。
基于入射暗场的HVM成像用于可视化SM-OM。首先，将SM分为四个不同区域；区域a（切牙-舌之间）、区域b（尖牙-第一前磨牙-舌之间）、区域c（第一-第二前磨牙-舌之间）、区域d（第二磨牙-智齿-舌之间）。其次，我们研究了颊部区域、下唇和上唇。比较各区域之间的总/功能血管密度（TVD/FCD）、焦深（FD）、小血管平均直径（SVMD）和毛细血管迂曲评分（CTS）。
招募了15名平均年龄为29±6岁的志愿者。在TVD（p = 0.30）、FCD（p = 0.38）和FD（p = 0.09）方面，舌下各区域之间未发现统计学差异。SVMD在区域a、区域b和区域c中相似（p = 0.20 - 0.30），在区域d中更大（p < 0.01，p = 0.015）。颊部区域的CTS高于唇部。
舌下区域在TVD、FCD、FD和SVMD方面具有均匀分布。本研究可为未来微循环评估研究提供正常微血管解剖结构的描述。
