白藜芦醇和氧化白藜芦醇对去铁胺诱导的人头颈鳞状细胞癌中癌症干细胞标志物表达的影响。
Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinoma.
作者信息
Amorntaveechai Artid, Osathanon Thanaphum, Pavasant Prasit, Sooampon Sireerat
机构信息
Interdepartmental Program of Pharmacology, Graduate School, Chulalongkorn University, Thailand.
Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Thailand.
出版信息
J Oral Biol Craniofac Res. 2022 Mar-Apr;12(2):253-257. doi: 10.1016/j.jobcr.2022.03.003. Epub 2022 Mar 16.
OBJECTIVES
Resveratrol and oxyresveratrol, a resveratrol derivative, possess various pharmacological activities, including anti-cancer activities. Because cancer stem cells play an important role in cancer recurrence, the aims of this study were to investigate whether resveratrol or oxyresveratrol can inhibit the expression of cancer stem cell markers under hypoxia.
MATERIALS AND METHODS
Deferoxamine was used to mimic the hypoxic condition. The mRNA expression of cancer stem cell markers was analyzed by Real-time PCR. Flow cytometry was used to determine the number of and cells.
RESULTS
Deferoxamine dose-dependently induced the expression of cancer stem cell markers; , , , and . The induction of these cancer stem cells markers was inhibited when the cells were treated with either resveratrol or oxyresveratrol. Moreover, we found that resveratrol also reduced the number of and cells after deferoxamine treatment.
CONCLUSIONS
Resveratrol and oxyresveratrol inhibit the expression of cancer stem cell markers and might target cancer stem cells in a hypoxia-associated tumor.
目的
白藜芦醇及其衍生物氧化白藜芦醇具有多种药理活性，包括抗癌活性。由于癌症干细胞在癌症复发中起重要作用，本研究旨在探讨白藜芦醇或氧化白藜芦醇在缺氧条件下是否能抑制癌症干细胞标志物的表达。
材料与方法
用去铁胺模拟缺氧条件。通过实时聚合酶链反应分析癌症干细胞标志物的mRNA表达。采用流式细胞术测定 和 细胞的数量。
结果
去铁胺剂量依赖性地诱导癌症干细胞标志物的表达； 、 、 、 和 。当细胞用白藜芦醇或氧化白藜芦醇处理时，这些癌症干细胞标志物的诱导受到抑制。此外，我们发现白藜芦醇还减少了去铁胺处理后 和 细胞的数量。
结论
白藜芦醇和氧化白藜芦醇抑制癌症干细胞标志物的表达，可能靶向缺氧相关肿瘤中的癌症干细胞。
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