白藜芦醇、氧化白藜芦醇和二羟基白藜芦醇对阿霉素处理的肺癌细胞的比较研究。
Comparative Studies of Resveratrol, Oxyresveratrol and Dihydrooxyresveratrol on Doxorubicin-Treated Lung Cancer Cells.
机构信息
Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand.
KMITL research and innovation services, King Mongkut's Institute of Technology Ladkrabang, Bangkok 100520, Thailand.
出版信息
Asian Pac J Cancer Prev. 2024 Mar 1;25(3):939-949. doi: 10.31557/APJCP.2024.25.3.939.
OBJECTIVES
This research aims to comparatively investigate the capability of resveratrol (RES) and RES analogues, oxyresveratrol (Oxy-RES) and dihydrooxyresveratrol (DHoxy-RES), to potentiate doxorubicin (DOX) effects against lung carcinoma epithelial cells.
METHODS
All experiments were performed on lung carcinoma cell lines (A549) with DOX combination between DOX and RES or RES analogues. Cell viability or growth inhibitory effect was assessed by MTT assay and genes associated with survival and metastasis were monitored by real-time polymerase chain reaction (RT-PCR).
RESULTS
DOX obviously demonstrated cytotoxic and anti-metastatic activities against A549 cells. Expression of gene-associated with both activities was potentiated by RES and RES analogues. Oxy-RES showed highest capability to potentiate DOX effects. DHoxy-RES showed nearly no effect to DOX activities.
CONCLUSIONS
These results provided an important basis of DOX combination with RES analogues, especially Oxy-RES, for better therapeutic effect. Further studies in human should be performed on exploring combination of DOX and Oxy-RES.
目的
本研究旨在比较研究白藜芦醇（RES）及其类似物，氧白藜芦醇（Oxy-RES）和二羟基白藜芦醇（DHoxy-RES）增强阿霉素（DOX）对肺癌上皮细胞作用的能力。
方法
在 DOX 与 RES 或 RES 类似物的组合中，在肺癌细胞系（A549）上进行了所有实验。通过 MTT 法评估细胞活力或生长抑制作用，并通过实时聚合酶链反应（RT-PCR）监测与存活和转移相关的基因。
结果
DOX 对 A549 细胞表现出明显的细胞毒性和抗转移活性。RES 和 RES 类似物增强了与这两种活性相关的基因的表达。Oxy-RES 显示出最强的增强 DOX 作用的能力。DHoxy-RES 对 DOX 活性几乎没有影响。
结论
这些结果为 DOX 与 RES 类似物（特别是 Oxy-RES）联合使用提供了重要的基础，以获得更好的治疗效果。应在人类中进行进一步的研究，以探索 DOX 和 Oxy-RES 的联合应用。
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