Hu Junmei, Xiao Guilian, Jiang Peng, Zhao Yan, Zhang Guangxu, Ma Xin, Yao Jie, Xue Lixia, Su Peisen, Bao Yinguang
State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, 271018, The People's Republic of China.
Lianyungang Academy of Agricultural Sciences, Lianyungang, 222000, The People's Republic of China.
BMC Plant Biol. 2022 Mar 21;22(1):129. doi: 10.1186/s12870-022-03523-x.
Wheat processing quality is an important factor in evaluating overall wheat quality, and dough characteristics are important when assessing the processing quality of wheat. As a notable germplasm resource, semi-wild wheat has a key role in the study of wheat processing quality.
In this study, four dough rheological characteristics were collected in four environments using a nested association mapping (NAM) population consisting of semi-wild and domesticated wheat varieties to identify quantitative trait loci (QTL) for wheat processing quality. A total of 49 QTL for wheat processing quality were detected, explaining 0.36-10.82% of the phenotypic variation. These QTL were located on all wheat chromosomes except for 2D, 3A, 3D, 6B, 6D and 7D. Compared to previous studies, 29 QTL were newly identified. Four novel QTL, QMlPH-1B.4, QMlPH-3B.4, QWdEm-1B.2 and QWdEm-3B.2, were stably identified in three or more environments, among which QMlPH-3B.4 was a major QTL. Moreover, eight important genetic regions for wheat processing quality were identified on chromosomes 1B, 3B and 4D, which showed pleiotropy for dough characteristics. In addition, out of 49 QTL, 15 favorable alleles came from three semi-wild parents, suggesting that the QTL alleles provided by the semi-wild parent were not utilized in domesticated varieties.
The results show that semi-wild wheat varieties can enrich the existing wheat gene pool and provide broader variation resources for wheat genetic research.
小麦加工品质是评估小麦整体品质的重要因素，而面团特性在评估小麦加工品质时至关重要。作为一种显著的种质资源，半野生小麦在小麦加工品质研究中具有关键作用。
在本研究中，利用由半野生和驯化小麦品种组成的巢式关联作图（NAM）群体，在四种环境中收集了四种面团流变学特性，以鉴定小麦加工品质的数量性状位点（QTL）。共检测到49个小麦加工品质QTL，解释了0.36%-10.82%的表型变异。这些QTL位于除2D、3A、3D、6B、6D和7D之外的所有小麦染色体上。与先前的研究相比，新鉴定出29个QTL。四个新的QTL，QMlPH-1B.4、QMlPH-3B.4、QWdEm-1B.2和QWdEm-3B.2，在三个或更多环境中被稳定鉴定，其中QMlPH-3B.4是一个主效QTL。此外，在1B、3B和4D染色体上鉴定出八个小麦加工品质的重要遗传区域，这些区域对面团特性表现出多效性。另外，在49个QTL中，有15个有利等位基因来自三个半野生亲本，这表明半野生亲本提供的QTL等位基因在驯化品种中未被利用。
结果表明，半野生小麦品种可以丰富现有的小麦基因库，并为小麦遗传研究提供更广泛的变异资源。
