Chhibber S, Vadehra D V
J Gen Microbiol. 1986 Apr;132(4):1051-4. doi: 10.1099/00221287-132-4-1051.
Klebocin, a bacteriocin produced by Klebsiella pneumoniae 158, was purified to homogeneity by ammonium sulphate fractionation and sequential DEAE-Sephacel and Sephadex G-150 column chromatography. The purified preparation had an Mr of approximately 40 000 on SDS-PAGE. Chemical analysis of the purified preparation showed it to be a protein, and it was sensitive to digestion by various proteolytic enzymes.
肺炎克雷伯菌158产生的细菌素klebocin，通过硫酸铵分级分离以及连续的DEAE-葡聚糖凝胶和葡聚糖凝胶G-150柱色谱法纯化至同质。纯化后的制剂在SDS-PAGE上的相对分子质量约为40000。对纯化制剂的化学分析表明它是一种蛋白质，并且对各种蛋白水解酶的消化敏感。
