Abrehem K, Zamiri I
J Gen Microbiol. 1985 Apr;131(4):707-13. doi: 10.1099/00221287-131-4-707.
Corynebacterium ulcerans strain 378 produces a bacteriocin (ulceracin 378) and a toxin when grown on semi-solid medium. Ulceracin 378 was purified 360-fold by dialysis and chromatography on DEAE-cellulose and Sephadex G-200. On the basis of Ultrogel AcA22 gel filtration its molecular weight was about 900 000. It could be dissociated by 2-mercaptoethanol and sodium dodecyl sulphate into smaller subunits of 25 000. The bactericidal activity was associated with this subunit which contained no carbohydrate or lipid. Ulceracin 378 was thermostable and stable over a wide pH range. Purified ulceracin 378 did not have a toxic effect (lethal) on guinea-pigs and rabbits and was immunologically distinct from the toxin.
溃疡棒状杆菌378菌株在半固体培养基上生长时会产生一种细菌素（溃疡菌素378）和一种毒素。通过透析以及在DEAE - 纤维素和葡聚糖G - 200上进行色谱分离，溃疡菌素378被纯化了360倍。基于超凝胶AcA22凝胶过滤，其分子量约为900000。它可被2 - 巯基乙醇和十二烷基硫酸钠解离成25000的较小亚基。杀菌活性与该不含碳水化合物或脂质的亚基相关。溃疡菌素378具有热稳定性，并且在很宽的pH范围内都稳定。纯化的溃疡菌素378对豚鼠和兔子没有毒性作用（致死性），并且在免疫学上与毒素不同。
