Holbach L, Hofmann C, Völcker H E, Naumann G O
Klin Monbl Augenheilkd. 1986 Aug;189(2):128-32. doi: 10.1055/s-2008-1050769.
Involucrin is a precursor of the cross-linked envelope protein of the human stratum corneum. Its appearance in the upper layers of the epidermis reflects normal differentiation of keratinocytes. This study uses an immunoperoxidase technique for localization of involucrin in paraffin sections of normal conjunctiva, conjunctival dysplasia, carcinoma in situ, and invasive carcinoma. Clinicoimmunocytochemical correlations are presented. The results demonstrate that the distribution patterns of involucrin differ in precancerous and cancerous conjunctival lesions: normal limbal conjunctiva shows involucrin only in the three superficial cell layers; the fornix conjunctivae contains no involucrin. All 23 conjunctival dysplasias show an involvement also of deeper layers of the epithelium, sparing the basal layers. Three carcinomas in situ and one invasive squamous cell carcinoma, however, demonstrate an involvement of all layers of the epithelium. The involucrin staining pattern helps in histologic differential diagnosis of epithelial lesions of the conjunctiva.
兜甲蛋白是人类角质层交联包膜蛋白的前体。它在表皮上层的出现反映了角质形成细胞的正常分化。本研究采用免疫过氧化物酶技术对正常结膜、结膜发育异常、原位癌和浸润癌的石蜡切片中的兜甲蛋白进行定位。并呈现了临床免疫细胞化学相关性。结果表明，兜甲蛋白在癌前和癌性结膜病变中的分布模式不同：正常角膜缘结膜仅在三个浅表细胞层显示兜甲蛋白；穹窿结膜不含兜甲蛋白。所有23例结膜发育异常均显示上皮深层也受累，基底层未受累。然而，3例原位癌和1例浸润性鳞状细胞癌显示上皮各层均受累。兜甲蛋白染色模式有助于结膜上皮病变的组织学鉴别诊断。
