Said J W, Sassoon A F, Shintaku I P, Banks-Schlegel S
J Invest Dermatol. 1984 May;82(5):449-52. doi: 10.1111/1523-1747.ep12260937.
Involucrin is a precursor of cross-linked protein of human stratum corneum, and its appearance in the upper layers of the epidermis is a function of the normal differentiation of the keratinocyte. Cases of basal cell and squamous cell carcinoma were evaluated for the presence of involucrin using immunoperoxidase techniques on paraffin sections. Basal cell carcinomas were negative for involucrin with staining restricted to squamous horn cysts, while squamous cell carcinomas stained strongly, particularly in large keratinized cells. Cases of squamous cell carcinoma in situ (Bowen's disease) revealed increased staining for involucrin with staining of dyskeratotic cells at all levels in the epithelium. Abnormal patterns of staining were also noted in non-neoplastic epidermis adjacent to carcinomas. Immunohistochemical staining for involucrin identifying abnormal or premature keratinization is a sensitive marker for dyskeratosis in squamous epithelia and may have applications in the histopathologic evaluation of skin specimens.
兜甲蛋白是人类角质层交联蛋白的前体，其在表皮上层的出现是角质形成细胞正常分化的一种表现。采用免疫过氧化物酶技术对石蜡切片上的基底细胞癌和鳞状细胞癌病例进行兜甲蛋白检测。基底细胞癌对兜甲蛋白呈阴性反应，染色仅限于鳞状角囊肿，而鳞状细胞癌则呈强阳性染色，尤其是在大的角化细胞中。原位鳞状细胞癌（鲍温病）病例显示兜甲蛋白染色增加，上皮各层的角化不良细胞均有染色。在癌旁的非肿瘤性表皮中也观察到异常的染色模式。通过免疫组织化学染色检测兜甲蛋白来识别异常或过早的角化，是鳞状上皮发育异常的一个敏感标志物，可能在皮肤标本的组织病理学评估中有应用价值。
