长链非编码 RNA PAXIP-AS1 通过 miR-210-3p/BDNF 轴促进 HTR-8/SVneo 细胞的活力、侵袭和迁移。
Long non-coding RNA PAXIP-AS1 promotes viability, invasion, and migration of HTR-8/SVneo cells through miR-210-3p/BDNF axis.
机构信息
Department of Gynecology and Obstetrics, Shengli Oilfield Central Hospital, Dongying City, Shandong Province, China.
Department of Reproductive Medicine, Shengli Oilfield Central Hospital, Dongying City, Shandong Province, China.
出版信息
Hypertens Pregnancy. 2022 May;41(2):107-115. doi: 10.1080/10641955.2022.2056194. Epub 2022 Mar 22.
OBJECTIVE
The aim of this research was to explore the role and potential mechanism of long non-coding RNA PAXIP-AS1 in preeclampsia.
METHODS
To investigate the effects of PAXIP-AS1 on cell viability, migration, and invasion. The miR-210-3p-targeted relationship with lncRNA PAXIP-AS1 or BDNF was verified.
RESULTS
PAXIP-AS1 was inversely correlated with miR-210-3p and BDNF was targeted by miR-210-3p. BDNF was positively correlated with PAXIP-AS1 in the serum of preeclampsia patients. The promotion effects of PAXIP-AS1 on cell viability, migration, and invasion were reversed by miR-210-3p up-regulation or BDNF knockdown in trophoblast cells.
CONCLUSION
PAXIP-AS1 promoted the viability, migration, and invasion of trophoblast cells by regulating the miR-210-3p/BDNF axis.
目的
本研究旨在探讨长链非编码 RNA PAXIP-AS1 在子痫前期中的作用及潜在机制。
方法
探讨 PAXIP-AS1 对细胞活力、迁移和侵袭的影响。验证 lncRNA PAXIP-AS1 或 BDNF 与 miR-210-3p 的靶向关系。
结果
PAXIP-AS1 与 miR-210-3p 呈负相关，BDNF 是 miR-210-3p 的靶基因。子痫前期患者血清中 BDNF 与 PAXIP-AS1 呈正相关。上调 miR-210-3p 或敲低 BDNF 可逆转 PAXIP-AS1 对滋养细胞活力、迁移和侵袭的促进作用。
结论
PAXIP-AS1 通过调节 miR-210-3p/BDNF 轴促进滋养细胞的活力、迁移和侵袭。
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