Department of Civil and Environmental Engineering, University of California, Los Angeles, CA 90095, United States.
Department of Civil and Environmental Engineering, University of California, Los Angeles, CA 90095, United States; Department of Civil and Environmental Engineering, University of Pittsburgh, Pittsburgh, PA 15260, United States.
Bioresour Technol. 2022 May;351:127040. doi: 10.1016/j.biortech.2022.127040. Epub 2022 Mar 19.
This study presents an eco-friendly and efficient technology, using immobilized enzymes, vault-encapsulated laccases (vlaccase), for decolorization and detoxification of dyes. Vault encapsulation remarkably improved the performance of laccase at industrially relevant conditions, including neutral to alkaline pH and relatively high temperatures. Two representative anthraquinone and azo dyes, Reactive Blue 19 and Acid Orange 7, respectively, were rapidly decolorized (72% and 80%) by vlaccase treatment while natural laccase (nlaccase) achieved 40% and 32% decolorization. The toxicity of treated and untreated dyes was tested on model bacterial, algal, and insect cells. The inhibitory effects of dyes towards selected bacteria were reduced in vlaccase-treated samples. The chlorophyll synthesis in algae was less inhibited by dyes after vlaccase treatment. Furthermore, the toxicity of dye degradation products to insect cells was significantly mitigated in the vlaccase group. Collectively, these results indicate that vlaccase is a stable and strong enzymatic system for removing dyes from waters.
本研究提出了一种环保且高效的技术，使用固定化酶—— vault 封装漆酶（vlaccase），用于染料的脱色和解毒。Vault 封装显著提高了漆酶在工业相关条件下的性能，包括中性到碱性 pH 值和相对较高的温度。两种代表性的蒽醌和偶氮染料，分别为活性蓝 19 和酸性橙 7，经 vlaccase 处理后迅速脱色（72%和 80%），而天然漆酶（nlaccase）的脱色率分别为 40%和 32%。用模型细菌、藻类和昆虫细胞测试了处理和未处理染料的毒性。在 vlaccase 处理的样品中，染料对选定细菌的抑制作用降低。在 vlaccase 处理后，藻类中叶绿素的合成受染料的抑制作用较小。此外，vlaccase 组显著减轻了染料降解产物对昆虫细胞的毒性。总之，这些结果表明，vlaccase 是一种从水中去除染料的稳定且强大的酶系统。
