用抗脂肪酸抗体对“衰老”大鼠皮质进行膜标记。
Membrane labeling of the cortex of 'aging' rats by anti-fatty acid antibodies.
作者信息
Maneta-Peyret L, Onteniente B, Geffard M, Cassagne C
出版信息
Neurosci Lett. 1986 Aug 29;69(2):121-5. doi: 10.1016/0304-3940(86)90589-6.
The cortex of 'aging' rat brain exhibits no significant difference in the lipid and fatty acid composition in comparison with that of control rat brain, except for a lowered value of the cholesterol/phospholipids ratio. However, an antiserum raised against oleic acid, specifically labels neurones of the cortex of the 'aging' rat mainly within layers IV and V. An electron microscopical study revealed that immunoreactivity was associated with cytoplasmic vesicular inclusions (lipofuscin) and with membranes. Thus, these anti-fatty acid antibodies may help in the evaluation of local modifications of membranes which are not predictable on the basis of biochemical analysis of lipids.
与对照大鼠脑相比，“衰老”大鼠脑皮质的脂质和脂肪酸组成无显著差异，只是胆固醇/磷脂比值有所降低。然而，用油酸制备的抗血清特异性地标记了“衰老”大鼠皮质主要位于IV层和V层内的神经元。电子显微镜研究显示，免疫反应性与细胞质囊泡内含物（脂褐素）及膜有关。因此，这些抗脂肪酸抗体可能有助于评估膜的局部修饰，而这些修饰是基于脂质生化分析无法预测的。
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