Carlier Y, Bout D, Fruchart J C, Desreumaux C, Dewailly P, Sezille G, Jaillard J
J Immunol Methods. 1978;21(3-4):317-24. doi: 10.1016/0022-1759(78)90158-8.
Inhibition enzyme immunoassay was applied to human apolipoprotein B (apo-B) from plasma. The technical conditions of the assay were determined. The detection limits of the assay were 200 ng to 10 microgram/ml. Correlation coefficients obtained between enzymoassay and rocket immunoelectrophoresis on one hand and radial immunodiffusion on the other were respectively 0.84 and 0.80. The inhibition enzymoassay provides a specific and highly sensitive method for the quantitation of apo-B.
抑制酶免疫测定法应用于血浆中的人载脂蛋白B（apo-B）。确定了该测定法的技术条件。该测定法的检测限为200纳克至10微克/毫升。酶测定法与火箭免疫电泳以及与单向免疫扩散法之间获得的相关系数分别为0.84和0.80。抑制酶测定法为apo-B的定量提供了一种特异且高度灵敏的方法。
