Fievet-Desreumaux C, Dedonder-Decoopman E, Fruchart J C, Dewailly P, Sezille G
Clin Chim Acta. 1979 Jul 16;95(2):405-8. doi: 10.1016/0009-8981(79)90384-x.
The Hyland laser nephelometer PDQ system for the assay of apolipoprotein B (apo-B) in human serum is described. Within and between-batch precision, accuracy and reliability are discussed. This instrument represents an important development in the immunochemical assay of apo-B, and the speed, precision, and convenience of the methodology make such a system attractive. Quantitation of apo-B was assessed in normal and hyperlipaemic subjects. Comparisons were made with two other specific and sensitive immunological methods for quantifying apo-B: enzymeimmunoassay (EIA) and rocket immunoelectrophoresis (RIE). Results obtained by the three methods correlated very well.
描述了用于检测人血清中载脂蛋白B（apo-B）的海兰德激光散射比浊法PDQ系统。讨论了批内和批间精密度、准确度及可靠性。该仪器代表了apo-B免疫化学检测的一项重要进展，该方法的速度、精密度和便利性使这样一个系统颇具吸引力。在正常和高脂血症受试者中评估了apo-B的定量。将其与另外两种用于定量apo-B的特异性和灵敏性免疫方法进行了比较：酶免疫测定法（EIA）和火箭免疫电泳法（RIE）。三种方法获得的结果相关性非常好。
