Department of Microbiology, University of Health Sciences, Lahore.
Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
J Pak Med Assoc. 2022 Feb;72(2):296-299. doi: 10.47391/JPMA.20-509.
To determine the susceptibility pattern of methicillin-resistant staphylococcus aureus to different antibiotics.
The descriptive study was conducted at the Microbiology Department of the University of Health Sciences, Lahore, Pakistan, from August 2016 to July 2019, and comprised staphylococcus aureus samples that were processed and identified using colony morphology on blood agar, gram stain, catalase, coagulase and deoxyribonuclease testing. Screening for methicillin-resistant staphylococcus aureus was done using cefoxitin disc 30µg and oxacillin disc 1mg. Antimicrobial susceptibility was tested using the modified Kirby-Bauer disc diffusion method in line with the Clinical and Laboratory Standards Institute guidelines 2019. Data was analysed using SPSS 24.
Of the 2704 strains processed, 402(14.86%) were found to be methicillin-resistant staphylococcus aureus. Of them, 204(50.74%) were recovered from pus, while 10(2.48%) were recovered from urine. All 402(100%) isolates were sensitive to vancomycin and linezolid, and resistant to penicillin, followed by erythromycin 306(76.11%) and sulfamethoxazole/ trimethoprim 295(73.38%). Overall, lower resistance was seen with doxycycline 145(36.06%) and clindamycin 160(39.80%). Inducible clindamycin resistance was seen in 142(35.23%) isolates.
An efficacious susceptibility pattern of methicillin-resistant staphylococcus aureus was seen with vancomycin and linezolid, moderate susceptibility with doxycycline and clindamycin, while high resistance was observed for penicillin, erythromycin and trimethoprim/sulfamethoxazole.
确定耐甲氧西林金黄色葡萄球菌对不同抗生素的敏感性模式。
本研究为描述性研究，于 2016 年 8 月至 2019 年 7 月在巴基斯坦拉合尔健康科学大学微生物学系进行，纳入使用血琼脂、革兰染色、触酶、凝固酶和脱氧核糖核酸酶试验进行处理和鉴定的金黄色葡萄球菌样本。使用头孢西丁 30μg 纸片和苯唑西林 1mg 纸片进行耐甲氧西林金黄色葡萄球菌的筛选。根据 2019 年临床和实验室标准协会指南，使用改良 Kirby-Bauer 纸片扩散法进行抗生素敏感性检测。采用 SPSS 24 进行数据分析。
在所处理的 2704 株菌株中，发现 402（14.86%）株为耐甲氧西林金黄色葡萄球菌。其中，204（50.74%）株从脓液中分离，10（2.48%）株从尿液中分离。所有 402（100%）株分离物均对万古霉素和利奈唑胺敏感，对青霉素耐药，其次是红霉素 306（76.11%）和磺胺甲噁唑/甲氧苄啶 295（73.38%）。总的来说，多西环素 145（36.06%）和克林霉素 160（39.80%）的耐药率较低。142（35.23%）株分离物表现出诱导型克林霉素耐药。
耐甲氧西林金黄色葡萄球菌对万古霉素和利奈唑胺的敏感性模式有效，对多西环素和克林霉素的敏感性适中，而对青霉素、红霉素和磺胺甲噁唑/甲氧苄啶的耐药性较高。
