Wiedemann Katja R, Peter Ventura Alejandra, Gerbig Stefanie, Roderfeld Martin, Quack Thomas, Grevelding Christoph G, Roeb Elke, Spengler Bernhard
Institute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Giessen, Germany.
Gastroenterology, Justus Liebig University Giessen, Giessen, Germany.
Anal Bioanal Chem. 2022 May;414(12):3653-3665. doi: 10.1007/s00216-022-04006-6. Epub 2022 Mar 23.
Schistosomiasis, caused by the human parasite Schistosoma mansoni, is one of the WHO-listed neglected tropical diseases (NTDs), and it has severe impact on morbidity and mortality, especially in Africa. Not only the adult worms but also their eggs are responsible for health problems. Up to 50% of the eggs produced by the female worms are not excreted with the feces but are trapped in the host tissue, such as the liver, where they provoke immune responses and a change in the lipid profile. We built up a database with 372 infection markers found in livers of S. mansoni-infected hamsters, using LC-MS/MS for identification, followed by statistical analysis. Most of them belong to the lipid classes of phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), and triglycerides (TGs). We assigned some of these markers to specific anatomical structures by applying high-resolution MALDI MSI to cryosections of hamster liver and generating ion images based on the marker list from the LC-MS/MS experiments. Furthermore, enrichment and depletion of several markers were visualized.
由人体寄生虫曼氏血吸虫引起的血吸虫病是世界卫生组织列出的被忽视的热带病之一，对发病率和死亡率有严重影响，尤其是在非洲。不仅成虫，其虫卵也会引发健康问题。雌虫产出的虫卵中，高达50%不会随粪便排出，而是被困在宿主组织中，如肝脏，在那里它们会引发免疫反应并改变脂质谱。我们建立了一个数据库，其中包含在曼氏血吸虫感染的仓鼠肝脏中发现的372种感染标志物，使用液相色谱-串联质谱法（LC-MS/MS）进行鉴定，随后进行统计分析。它们中的大多数属于磷脂酰胆碱（PCs）、磷脂酰乙醇胺（PEs）和甘油三酯（TGs）等脂质类别。通过将高分辨率基质辅助激光解吸电离质谱成像（MALDI MSI）应用于仓鼠肝脏冷冻切片，并根据LC-MS/MS实验的标志物列表生成离子图像，我们将其中一些标志物分配到特定的解剖结构中。此外，还可视化了几种标志物的富集和消耗情况。
