Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center Rotterdam, Rotterdam, the Netherlands.
Biomolecular Mass Spectrometry & Proteomics, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands.
Mol Biochem Parasitol. 2020 Jul;238:111296. doi: 10.1016/j.molbiopara.2020.111296. Epub 2020 Jun 27.
Eggs, schistosomula and adult Schistosoma worms are known to release extracellular vesicles (EV) during in vitro incubations and these EVs are postulated to affect the host responses. So far only those EVs released during in vitro incubations of schistosomes have been studied and it is unknown whether in blood of infected hosts the schistosomal EVs can be detected amidst all the circulating EVs of the host itself. In this study we analyzed the protein as well as the phospholipid composition of EVs circulating in blood plasma of S. mansoni infected hamsters and compared those with the EVs circulating in blood of non-infected hamsters. Although neither proteins nor lipids specific for schistosomes could be detected in the circulating EVs of the infected hamsters, the infection with schistosomes had a marked effect on the circulating EVs of the host, as the protein as well as the lipid composition of EVs circulating in infected hamsters were different from the EVs of uninfected hamsters. The observed changes in the EV lipid and protein content suggest that more EVs are released by the diseased liver, the affected erythrocytes and activated immune cells.
已知鸡蛋、尾蚴和成虫期血吸虫在体外孵育过程中会释放细胞外囊泡（EV），这些 EV 被认为会影响宿主的反应。到目前为止，仅研究了在血吸虫体外孵育过程中释放的那些 EV，尚不清楚在感染宿主的血液中，血吸虫 EV 是否能在宿主自身所有循环的 EV 中被检测到。在这项研究中，我们分析了曼氏血吸虫感染仓鼠血浆中循环的 EV 的蛋白质和磷脂组成，并将其与非感染仓鼠血液中循环的 EV 进行了比较。尽管在感染仓鼠的循环 EV 中未检测到针对血吸虫的特异性蛋白质或脂质，但感染血吸虫对宿主循环 EV 有明显影响，因为感染仓鼠的 EV 的蛋白质和脂质组成与未感染仓鼠的 EV 不同。观察到 EV 脂质和蛋白质含量的变化表明，患病肝脏、受影响的红细胞和激活的免疫细胞释放了更多的 EV。
