Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.
Department of Biology, Faculty of Science and Arts, Al-Baha University, Almakhwah, Al-Baha, Saudi Arabia.
Environ Sci Pollut Res Int. 2022 Aug;29(37):55790-55802. doi: 10.1007/s11356-022-19747-1. Epub 2022 Mar 23.
Ulcerative colitis (UC) is a chronic autoimmune inflammatory disease associated with extensive mucosal damage. Prodigiosins (PGs) are natural bacterial pigments with well-known antioxidant and immunosuppressive properties. In the current study, we examined the possible protective effect of PGs loaded with selenium nanoparticles (PGs-SeNPs) against acetic acid (AcOH)-induced UC in rats. Thirty-five rats were separated into five equal groups with seven animals/group: control, UC, PGs (300 mg/kg), sodium selenite (NaSeO, 2 mg/kg), PGs-SeNPs (0.5 mg/kg), and 5-aminosalicylates (5-ASA, 200 mg/kg). Interestingly, PGs-SeNPs administration lessened colon inflammation and mucosal damage as indicated by inhibiting inflammatory markers upon AcOH injection. Furthermore, PGs-SeNPs improved the colonic antioxidant capacity and prevented oxidative insults as evidenced by the upregulation of Nrf2- and its downstream antioxidants along with the decreased pro-oxidants [reactive oxygen species (ROS), carbonyl protein, malondialdehyde (MDA), inducible nitric oxide synthase (iNOS), and nitric oxide (NO] in the colon tissue. Furthermore, PGs-SeNPs protected intestinal cell loss through blockade apoptotic cascade by decreasing pro-apoptotic proteins [Bcl-2-associated X protein (Bax) and caspase-3] and increasing anti-apoptotic protein, B cell lymphoma 2 (Bcl2). Collectively, PGs-SeNPs could be used as an alternative anti-colitic option due to their strong anti-inflammatory, antioxidant, and anti-apoptotic activities.
溃疡性结肠炎（UC）是一种与广泛黏膜损伤相关的慢性自身免疫性炎症性疾病。灵菌红素（PGs）是具有明显抗氧化和免疫抑制特性的天然细菌色素。在本研究中，我们研究了载硒纳米颗粒的灵菌红素（PGs-SeNPs）对乙酸（AcOH）诱导的大鼠 UC 的可能保护作用。将 35 只大鼠分为 5 组，每组 7 只：对照组、UC 组、PGs（300mg/kg）组、亚硒酸钠（NaSeO，2mg/kg）组、PGs-SeNPs（0.5mg/kg）组和 5-氨基水杨酸（5-ASA，200mg/kg）组。有趣的是，PGs-SeNPs 给药减轻了结肠炎症和黏膜损伤，这表明在 AcOH 注射后抑制了炎症标志物。此外，PGs-SeNPs 改善了结肠抗氧化能力，并防止了氧化损伤，这表现为 Nrf2 及其下游抗氧化剂的上调，以及结肠组织中促氧化剂 [活性氧（ROS）、羰基蛋白、丙二醛（MDA）、诱导型一氧化氮合酶（iNOS）和一氧化氮（NO）] 的减少。此外，PGs-SeNPs 通过减少促凋亡蛋白[B 细胞淋巴瘤 2 相关 X 蛋白（Bax）和半胱天冬酶-3]和增加抗凋亡蛋白 B 细胞淋巴瘤 2（Bcl2）来阻止凋亡级联反应，从而保护肠细胞丢失。总之，PGs-SeNPs 由于具有较强的抗炎、抗氧化和抗凋亡活性，可作为一种替代的抗结肠炎选择。
