Departamento de Análises Clínicas, Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brasil.
Departamento de Radiofarmácia, Instituto de Pesquisas Energéticas e Nucleares, IPEN/CNEN, São Paulo, SP, Brasil.
Methods Mol Biol. 2022;2442:339-352. doi: 10.1007/978-1-0716-2055-7_18.
Molecular imaging (MI) is a non-invasive growing technology that allows the investigation of cellular and molecular processes in basic and clinical research and medicine. Luminescent proteins and radionuclides can be associated to target molecules providing high-definition and real-time image of whole body in few minutes or hours. Several MI studies have enabled the determination of molecular partners, in vivo tracking, and fate of compounds in different disorders. Considering that galectins are multifaceted proteins with great impact in many biological events, here we describe methods and strategies to generate labeled galectins for in vivo non-invasive imaging studies.
分子影像学（MI）是一种非侵入性的新兴技术，可用于基础和临床研究以及医学领域中对细胞和分子过程的研究。发光蛋白和放射性核素可以与靶分子结合，在几分钟或几小时内提供全身的高清晰度实时图像。多项 MI 研究已经能够确定分子伴侣，在体内跟踪化合物在不同疾病中的命运。鉴于半乳糖凝集素是具有重大影响的多功能蛋白，在许多生物学事件中，在这里我们描述了用于体内非侵入性成像研究的标记半乳糖凝集素的方法和策略。
