使用鸡胚尿囊膜试验研究半乳糖凝集素在体内血管生成中的作用的方法。

Method to Study the Role of Galectins in Angiogenesis In Vivo Using the Chick Chorioallantoic Membrane Assay.

机构信息

Amsterdam UMC location VUmc, Department of Radiation Oncology, Cancer Center Amsterdam, Amsterdam, The Netherlands.

出版信息

Methods Mol Biol. 2022;2442:621-633. doi: 10.1007/978-1-0716-2055-7_33.

DOI:10.1007/978-1-0716-2055-7_33

PMID:35320549

Abstract

Angiogenesis is a complex multi-step process involving various activities of endothelial cells. These activities are influenced in vivo by environmental conditions like interactions with other cell types and the microenvironment. Galectins play a role in several of these interactions and are therefore required for proper execution of in vivo angiogenesis. This chapter describes a method to study galectins during physiologic and pathophysiologic angiogenesis in vivo using the chicken chorioallantoic membrane (CAM) assay.

摘要

血管生成是一个涉及内皮细胞各种活动的复杂多步骤过程。这些活动在体内受到环境条件的影响，如与其他细胞类型的相互作用和微环境。半乳糖凝集素在其中的一些相互作用中发挥作用，因此对于体内血管生成的正常执行是必需的。本章描述了一种使用鸡胚尿囊膜（CAM）测定法在体内研究生理和病理血管生成过程中半乳糖凝集素的方法。