Pomona College, Claremont, CA, USA.
Methods Mol Biol. 2022;2468:25-41. doi: 10.1007/978-1-0716-2181-3_2.
Caenorhabditis elegans is an excellent organism for teaching or doing short-term research projects because of the many freely-available comprehensive resources describing its genome and biology, its short-generation time and the ease of working with it in the lab. However, it can be daunting to begin a short-term project or a teaching lab with an organism with which one has little or no experience. Therefore, in this article, we will discuss the minimal equipment, reagents, and protocols needed to begin doing experiments with C. elegans as well as inclusive teaching practices. We will also describe a teaching laboratory for introductory or advanced neuroscience undergraduate courses where students learn to understand the relationship between genetic mutations and behavior through using chemotaxis assays. In addition, instructions for students for the lab module are provided in Subheading "Example of Teaching Lab Module for Students".
秀丽隐杆线虫是一种极好的教学或短期研究项目的生物，因为有许多免费的综合资源描述了它的基因组和生物学特性，它的世代时间短，并且在实验室中很容易进行操作。然而，如果一个人对一个几乎没有或完全没有经验的生物开始一个短期项目或教学实验室，可能会让人望而却步。因此，在本文中，我们将讨论开始用秀丽隐杆线虫进行实验所需的最小设备、试剂和方案，以及包容性的教学实践。我们还将描述一个用于介绍性或高级神经科学本科课程的教学实验室，学生通过使用趋化性测定来学习理解基因突变与行为之间的关系。此外，在“学生教学实验室模块示例”中为学生提供了实验室模块的说明。
