Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USA.
Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Methods Mol Biol. 2022;2468:117-131. doi: 10.1007/978-1-0716-2181-3_7.
Optogenetic approaches have proven to be powerful for examining the roles of specific neurons in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity while quantitatively assessing behavior and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in feeding behavior.
光遗传学方法已被证明在研究特定神经元在产生行为中的作用方面非常有效，特别是在电生理操作不可行的系统中。在这里，我们描述了一种在完整的秀丽隐杆线虫中光遗传操纵单个咽神经元同时监测咽行为的方法。这种方法提供了对咽神经活动的双向和动态控制，同时定量评估行为，并使我们能够测试关于单个咽神经元在摄食行为中的作用的假设。
