Trojanowski Nicholas F, Fang-Yen Christopher
Department of Bioengineering, University of Pennsylvania, 210 S. 33rd Street, Philadelphia, PA, 19104, USA.
Department of Neuroscience, University of Pennsylvania, CRB 211, 415 Curie Boulevard, Philadelphia, PA, 19104, USA.
Methods Mol Biol. 2015;1327:105-19. doi: 10.1007/978-1-4939-2842-2_9.
Optogenetic approaches have proven powerful for examining the role of neural circuits in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity simultaneously with a behavioral readout and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in regulating feeding behavior.
光遗传学方法已被证明在研究神经回路在产生行为中的作用方面非常强大，特别是在无法进行电生理操作的系统中。在这里，我们描述了一种在完整的秀丽隐杆线虫中对单个咽部神经元进行光遗传学操纵，同时监测咽部行为的方法。这种方法能够在获得行为读数的同时，对咽部神经活动进行双向和动态控制，使我们能够测试关于单个咽部神经元在调节进食行为中作用的假设。
