Department of Chemistry, Division of Applied Physical Chemistry, KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Stockholm, Sweden.
Department of Industrial Biotechnology, KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Stockholm, Sweden.
Biotechnol J. 2022 Aug;17(8):e2100325. doi: 10.1002/biot.202100325. Epub 2022 May 13.
The increased use of biopharmaceuticals calls for improved means of bioprocess monitoring. In this work, capillary electrophoresis (CE) and microchip electrophoresis (MCE) methods were developed and applied for the analysis of amino acids (AAs) in cell culture supernatant. In samples from different days of a Chinese hamster ovary cell cultivation process, all 19 proteinogenic AAs containing primary amine groups could be detected using CE, and 17 out of 19 AAs using MCE. The relative concentration changes in different samples agreed well with those measured by high-performance liquid chromatography (HPLC). Compared to the more commonly employed HPLC analysis, the CE and MCE methods resulted in faster analysis, while significantly lowering both the sample and reagent consumption, and the cost per analysis.
生物制药的应用日益广泛，因此需要改进生物工艺监测手段。本研究中，开发并应用毛细管电泳（CE）和微芯片电泳（MCE）方法分析细胞培养上清液中的氨基酸（AA）。在一个中国仓鼠卵巢细胞培养过程中不同天数的样本中，CE 可检测到所有含有伯胺基团的 19 种蛋白源氨基酸，MCE 则可检测到 17 种。不同样本中相对浓度变化与高效液相色谱（HPLC）测量结果一致。与更常用的 HPLC 分析相比，CE 和 MCE 方法分析速度更快，同时显著降低了样品和试剂消耗，以及每次分析的成本。
