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抗氧化剂对暴露于颗粒物和香烟烟雾提取物的支气管上皮细胞氧化应激和自噬的影响。

Effects of Antioxidant on Oxidative Stress and Autophagy in Bronchial Epithelial Cells Exposed to Particulate Matter and Cigarette Smoke Extract.

作者信息

Hur Jung, Rhee Chin Kook, Jo Yong Suk

机构信息

Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.

出版信息

Tuberc Respir Dis (Seoul). 2022 Jul;85(3):237-248. doi: 10.4046/trd.2021.0152. Epub 2022 Mar 23.

DOI:10.4046/trd.2021.0152
PMID:35320665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9263338/
Abstract

BACKGROUND

We evaluated the effect of particulate matter (PM) and cigarette smoke extract (CSE) on bronchial epithelial cell survival, as well as oxidative stress and autophagy levels. Moreover, we aimed to assess the effect of the antioxidant N-acetylcysteine (NAC) on the adverse effects of PM and CSE exposure.

METHODS

Normal human bronchial epithelial cells (BEAS-2B cells) were exposed to urban PM with or without CSE, after which cytotoxic effects, including oxidative stress and autophagy levels, were measured. After identifying the toxic effects of urban PM and CSE exposure, the effects of NAC treatment on cell damage were evaluated.

RESULTS

Urban PM significantly decreased cell viability in a concentration-dependent manner, which was further aggravated by simultaneous treatment with CSE. Notably, pretreatment with NAC at 10 mM for 1 hour reversed the cytotoxic effects of PM and CSE co-exposure. Treatment with 1, 5, and 10 mM NAC was shown to decrease reactive oxygen species levels induced by exposure to both PM and CSE. Additionally, the autophagy response assessed via LC3B expression was increased by PM and CSE exposure, and this also attenuated by NAC treatment.

CONCLUSION

The toxic effects of PM and CSE co-exposure on human bronchial epithelial cells, including decreased cell viability and increased oxidative stress and autophagy levels, could be partly prevented by NAC treatment.

摘要

背景

我们评估了颗粒物（PM）和香烟烟雾提取物（CSE）对支气管上皮细胞存活、氧化应激和自噬水平的影响。此外，我们旨在评估抗氧化剂N-乙酰半胱氨酸（NAC）对PM和CSE暴露的不良影响。

方法

将正常人支气管上皮细胞（BEAS-2B细胞）暴露于含或不含CSE的城市PM中，之后测量细胞毒性作用，包括氧化应激和自噬水平。在确定城市PM和CSE暴露的毒性作用后，评估NAC处理对细胞损伤的影响。

结果

城市PM以浓度依赖性方式显著降低细胞活力，同时用CSE处理会使其进一步加剧。值得注意的是，用10 mM NAC预处理1小时可逆转PM和CSE共同暴露的细胞毒性作用。结果显示，用1、5和10 mM NAC处理可降低暴露于PM和CSE所诱导的活性氧水平。此外，通过LC3B表达评估的自噬反应在PM和CSE暴露后增加，并且这也通过NAC处理而减弱。

结论

NAC处理可部分预防PM和CSE共同暴露对人支气管上皮细胞的毒性作用，包括细胞活力降低以及氧化应激和自噬水平增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/2f19ca5562d8/trd-2021-0152f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/fbd44b228d9d/trd-2021-0152f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/902b5b89630b/trd-2021-0152f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/555d90362be8/trd-2021-0152f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/f80511ec03d7/trd-2021-0152f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/743cbf3f2f52/trd-2021-0152f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/3fa0fcb721eb/trd-2021-0152f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/2f19ca5562d8/trd-2021-0152f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/fbd44b228d9d/trd-2021-0152f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/902b5b89630b/trd-2021-0152f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/555d90362be8/trd-2021-0152f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/f80511ec03d7/trd-2021-0152f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/743cbf3f2f52/trd-2021-0152f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/3fa0fcb721eb/trd-2021-0152f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d388/9263338/2f19ca5562d8/trd-2021-0152f7.jpg

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