Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Department of Pharmacology & Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Pediatr Res. 2022 Dec;92(6):1575-1579. doi: 10.1038/s41390-022-02023-w. Epub 2022 Mar 23.
Anemia is a nearly universal diagnosis in preterm infants, caused by phlebotomy, and exacerbated by the underlying erythropoietic immaturity. Newborn infants are exposed to the unique stressor of fetal-to-neonatal transition, which requires significant adaptation ex utero. Accordingly, the preterm infant's response to anemia may alter the ability to confront underlying illness. This study utilized our preclinical mouse model of phlebotomy-induced anemia (PIA) to comprehensively investigate associated hematological changes.
C57BL/6 mice were subjected to timed phlebotomy between postnatal days 2--10 to induce severe anemia. Complete blood counts were determined by the Sysmex XT-2000iV analyzer.
Anemic pups showed a gradual reduction of RBC and hemoglobin (Hb) and increased reticulocyte (RET) counts and red cell distribution width (RDW), however, with reduced RET-Hb from postnatal day (P) of 4 onwards. Elevated levels of high fluorescent RET and immature reticulocyte fraction (IRF) were noted in anemic mouse pups, but low and medium fluorescent RET were reduced. Also, the reduction of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were noted in anemic pups. No changes were seen in lymphocytes, but monocytes and neutrophils were significantly elevated from P4-P6.
PIA in mouse pups is associated with hematological changes that may be exacerbating factors in neonatal diseases.
Anemia is common and often severe in premature infants. Investigation of hematological parameters in settings of preclinical anemia may be an index of therapeutic strategies. Preclinical model evaluating the effects of neonatal anemia on the remainder of complete blood count. Detailed time kinetic phlebotomy-induced anemic mice enable us to study the impact on developmental delays in erythropoiesis and possible strategic intervention. Hematological effects of severe anemia in mice might provide insight on how best to investigate anemia in preterm infants.
贫血是早产儿几乎普遍存在的诊断结果，由采血引起，并因红细胞生成不成熟而加重。新生儿面临胎儿到新生儿过渡的独特应激源，这需要在子宫外进行重大适应。因此，早产儿对贫血的反应可能会改变其应对潜在疾病的能力。本研究利用我们的采血诱导贫血（PIA）临床前小鼠模型全面研究相关的血液学变化。
C57BL/6 小鼠在出生后第 2-10 天接受定时采血以诱导严重贫血。通过 Sysmex XT-2000iV 分析仪测定全血细胞计数。
贫血幼鼠的 RBC 和血红蛋白（Hb）逐渐减少，网织红细胞（RET）计数和红细胞分布宽度（RDW）增加，但从第 4 天开始，RET-Hb 减少。贫血小鼠幼鼠的高荧光 RET 和未成熟网织红细胞分数（IRF）水平升高，但低荧光和中荧光 RET 减少。此外，贫血幼鼠的平均红细胞体积（MCV）、平均红细胞血红蛋白（MCH）和平均红细胞血红蛋白浓度（MCHC）减少。淋巴细胞无变化，但单核细胞和中性粒细胞从第 4-6 天明显升高。
在小鼠幼鼠中，PIA 与血液学变化相关，这些变化可能是新生儿疾病的加重因素。
贫血在早产儿中很常见且通常较为严重。在临床前贫血情况下对血液学参数的研究可能是治疗策略的指标。评估新生儿贫血对全血细胞计数其余部分影响的临床前模型。详细的时间动力学采血诱导贫血小鼠使我们能够研究对红细胞生成发育延迟的影响和可能的策略干预。严重贫血对小鼠的血液学影响可能为如何最好地研究早产儿贫血提供见解。
