基于金纳米颗粒的胶体金免疫层析法，使用手持扫描设备作为读取器，用于现场检测莠去津。

A gold nanoparticle-based lateral flow immunoassay for atrazine point-of-care detection using a handhold scanning device as reader.

机构信息

Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment & Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, China.

School of Public Health, Inner Mongolia Medical University, Hohhot, 010000, China.

出版信息

Mikrochim Acta. 2022 Mar 23;189(4):153. doi: 10.1007/s00604-021-05146-9.

DOI:10.1007/s00604-021-05146-9

PMID:35322310

Abstract

A method is described to achieve accurate quantitative detection of atrazine (ATZ) in maize by using lateral flow strips based on gold nanoparticles (GNPs) and a handheld scanning reader. GNPs of 15 nm in diameter were applied as label, and a lateral flow immune assay strip was prepared. The linear range was 5.01-95.86 ng mL with a detection limit of 4.92 ng mL in phosphate buffer, 4 times better than the readout by the naked eye. ATZ-spiked corn samples were also analysed. The accuracy of results of spiked samples was confirmed by ELISA and liquid chromatography-tandem mass spectrometry (HPLC), which proved the reliability of the proposed method. A handhold device with an optical scanning system was designed for on-site quantitative detection. Combined with the pretreatment, the assay could be completed in less than 20 min.

摘要

描述了一种使用基于纳米金（GNPs）的侧向流条带和手持式扫描读取器实现对玉米中莠去津（ATZ）进行准确定量检测的方法。将 15nm 直径的 GNPs 用作标记，并制备了侧向流免疫检测条带。在磷酸盐缓冲液中的线性范围为 5.01-95.86ng/mL，检测限为 4.92ng/mL，比肉眼读数好 4 倍。还分析了添加 ATZ 的玉米样品。通过酶联免疫吸附测定（ELISA）和液相色谱-串联质谱（HPLC）验证了加标样品结果的准确性，证明了该方法的可靠性。设计了一种带有光学扫描系统的手持式设备用于现场定量检测。结合预处理，整个检测过程可在 20 分钟内完成。