基于双重金纳米粒子结合物的侧向流动免疫分析中的信号增强。
Signal enhancement in a lateral flow immunoassay based on dual gold nanoparticle conjugates.
机构信息
College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, Hunan, China.
出版信息
Clin Biochem. 2013 Nov;46(16-17):1734-8. doi: 10.1016/j.clinbiochem.2013.08.010. Epub 2013 Aug 28.
OBJECTIVE
In order to amplify signal of lateral flow immunoassay for specific detection of thrombin.
DESIGN AND METHODS
A new, simple method of amplifying signals using two gold nanoparticle conjugates (GNP) in gold-nanoparticle-based lateral flow immunoassay without an additional step was developed. The first conjugates were prepared by labeling DNA1 with 30 nm GNPs, and the second conjugates were prepared by immobilizing both DNA2 and thrombin aptamer on the surfaces of 16 nm GNPs.
RESULTS
The detection limit was improved 30 times. The lateral flow immunoassay developed in this study was applied to detect thrombin concentration in the range of 0.5-120 nM with a detection limit of 0.25 nM.
CONCLUSIONS
The lateral flow immunoassay developed in this study was used to detect thrombin concentrations within a range of 0.5-120 nM with a detection limit of 0.25 nM. This assay is very versatile and can be easily extended to other proteins.
目的
为了增强侧向流动免疫分析（用于特异性检测凝血酶）的信号。
设计与方法
开发了一种新的、简单的信号放大方法，在金纳米粒子侧向流动免疫分析中使用两种金纳米粒子缀合物（GNP），无需额外步骤。第一种缀合物是通过用 30nm GNP 标记 DNA1 制备的，第二种缀合物是通过将 DNA2 和凝血酶适体固定在 16nm GNP 表面上制备的。
结果
检测限提高了 30 倍。本研究开发的侧向流动免疫分析用于检测 0.5-120 nM 范围内的凝血酶浓度，检测限为 0.25 nM。
结论
本研究开发的侧向流动免疫分析用于检测 0.5-120 nM 范围内的凝血酶浓度，检测限为 0.25 nM。该测定方法非常通用，可轻松扩展到其他蛋白质。
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