使用胶体金-抗体探针的免疫层析法检测水样中的莠去津。
Immunochromatography using colloidal gold-antibody probe for the detection of atrazine in water samples.
作者信息
Shim Won-Bo, Yang Zheng-You, Kim Ji-Young, Choi Jin-Gil, Je Jung-Hyun, Kang Sung-Jo, Kolosova Anna Yu, Eremin Sergei A, Chung Duck-Hwa
机构信息
Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea.
出版信息
J Agric Food Chem. 2006 Dec 27;54(26):9728-34. doi: 10.1021/jf0620057.
An immunochromatography (ICG) strip test for rapid detection of atrazine in water samples was developed. A monoclonal antibody (MAb) specific to atrazine was produced from the cloned hybridoma cell (AT-1-M3) and used to develop a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and ICG strip. MAb conjugated to colloidal gold, and that was applied to the conjugate pad of the ICG strip. The visual detection limit for the ICG strip was 3 ng/mL. This test required only 10 min to get results and one step of sample to perform the assay. The results of water samples spiked with 5, 10, 20, and 50 ng/mL of atrazine by ICG strip were in good agreement with those obtained by DC-ELISA. The ICG strip was sufficiently sensitive and accurate to be useful for rapid screening of atrazine in various water samples.
开发了一种用于快速检测水样中莠去津的免疫层析（ICG）试纸条检测方法。从克隆的杂交瘤细胞（AT-1-M3）中制备了对莠去津具有特异性的单克隆抗体（MAb），并用于开发直接竞争酶联免疫吸附测定（DC-ELISA）和ICG试纸条。与胶体金偶联的单克隆抗体被应用于ICG试纸条的结合垫。ICG试纸条的目视检测限为3 ng/mL。该检测仅需10分钟即可获得结果，且只需一步加样即可进行测定。用ICG试纸条对添加了5、10、20和50 ng/mL莠去津的水样进行检测的结果与用DC-ELISA获得的结果高度一致。ICG试纸条具有足够的灵敏度和准确性，可用于快速筛查各种水样中的莠去津。
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