Department of Obstetrics and Gynecology and Artificial Reproductive Techniques, Faculty of Medicine, Sakarya University, Serdivan, Sakarya, Turkey.
Department of Histology and Embryology and Artificial Reproductive Techniques, Faculty of Medicine, Sakarya University, Serdivan, Sakarya, Turkey.
J Obstet Gynaecol Res. 2022 Jun;48(6):1390-1398. doi: 10.1111/jog.15232. Epub 2022 Mar 23.
Ovarian torsion is a common cause of local ischemic damage, reduced follicular activity and infertility. This study aimed to investigate how well platelet-rich plasma (PRP) protects against experimental ischemic (I) and ischemia-reperfusion (I/R) injury in rat ovaries and its effect on in vitro fertilization (IVF) outcomes.
Fifty-six adult female Sprague-Dawley albino rats were randomly assigned to six groups of eight animals each: Sham, Ischemia, I/R, Sham + PRP, I + PRP, and I/R + PRP. The remaining eight animals were used to prepare the PRP. The ischemia groups were subjected to bilateral adnexal torsion for 3 h, while the I/R and I/R + PRP groups received subsequent detorsion for 3 h. Intraperitoneal (i.p.) PRP was administered 30 min prior to ischemia (I + PRP) or reperfusion (I/R + PRP). The ovaries were stimulated through an intraperitoneal injection of 150-300 internal units of IU/kg PMSG. After ovulation induction, oocytes were taken from the ovaries, and IVF was performed.
The number of MII oocytes reached the highest number with 4.63 ± 0.74 in the S group and had the lowest number with 0.50 ± 0.53 in the I/R group. There were statistically significant differences for the number of embryos obtained on the second day between the I and I + PRP groups and the I/R and I/R + PRP groups (p = 0.000). In comparing anti-Müllerian hormone 1 (AMH1) and AMH2 values within the group, the highest decrease was observed in the I and I/R groups.
PRP is effective in minimizing ovarian damage and preserving ovarian reserves following ovarian torsion.
卵巢扭转是局部缺血性损伤、卵泡活动减少和不孕的常见原因。本研究旨在探讨富血小板血浆（PRP）在多大程度上能预防大鼠卵巢的实验性缺血（I）和缺血再灌注（I/R）损伤，以及其对体外受精（IVF）结果的影响。
将 56 只成年雌性 Sprague-Dawley 白化大鼠随机分为六组，每组 8 只：假手术组（Sham）、缺血组（Ischemia）、缺血再灌注组（I/R）、假手术+PRP 组（Sham+PRP）、缺血+PRP 组（I+PRP）和缺血再灌注+PRP 组（I/R+PRP）。其余 8 只动物用于制备 PRP。缺血组双侧附件扭转 3 小时，而 I/R 和 I/R+PRP 组在随后的 3 小时内进行再灌注。在缺血（I+PRP）或再灌注（I/R+PRP）前 30 分钟，经腹腔内（i.p.）给予 PRP。通过腹腔内注射 150-300 国际单位/千克 PMSG 刺激卵巢排卵。排卵诱导后，从卵巢中取出卵母细胞并进行体外受精。
MII 卵母细胞的数量在 S 组中达到最高，为 4.63±0.74，在 I/R 组中达到最低，为 0.50±0.53。I 组和 I+PRP 组与 I/R 组和 I/R+PRP 组之间获得的第二天胚胎数量有统计学差异（p=0.000）。在比较组内抗苗勒管激素 1（AMH1）和 AMH2 值时，I 组和 I/R 组观察到最大的下降。
PRP 可有效减轻卵巢扭转后卵巢损伤，保护卵巢储备。
