Sakarya Üniversitesi, Faculty of Medicine, Department of Obstetrics and Gynecology and Artificial Reproductive Techniques - Serdivan, Turkey.
Sakarya Üniversitesi, Faculty of Medicine, Department of Histology and Embryology and Artificial Reproductive Techniques - Serdivan, Turkey.
Rev Assoc Med Bras (1992). 2023 Feb 20;69(3):380-385. doi: 10.1590/1806-9282.20220385. eCollection 2023.
The study aimed to investigate the protection of enoxaparin (E) against experimental ischemic (I) and ischemic-reperfusion (I/R) injury in rat ovaries on in vitro fertilization outcomes.
In total, 56 adult female Sprague-Dawley albino rats were randomly assigned to 6 groups of 8 animals each: Sham, Ischemia, I/R, Sham+E, I+E, and I/R+E. Ischemia groups were subjected to bilateral adnexal torsion for 3 h. In contrast, I/R and I/R+E groups received subsequent detorsion for 3 h. Enoxaparin (0.5 mg/kg s.c.) was administered 30 min prior to ischemia (I+platelet-rich plasma) or reperfusion (I/R+I+platelet-rich plasma). Ovaries were stimulated through intraperitoneal injection of 150-300 internal units IU/kg pregnant mare serum gonadotropin. Anti-Müllerian hormone levels were measured before and after surgery in all groups.
When the number of metaphase II oocytes was evaluated, statistically significant differences were observed between the I and I+E (p=0.001) and I/R and I/R+E (p=0.000) groups. When both I and I+E groups and I/R and I/R+E groups were compared, it was found that E application increased the number of fertilized oocytes. The number of embryos on the second day was higher in the I/R+E group than that in the I/R group. Statistically significant differences were found in the number of grade 1 embryos between the I/R and I/R+E groups (p=0.003). In comparing anti-Müllerian hormone values within the group, the highest decrease was observed in the I and I/R groups.
Enoxaparin effectively minimizes ovarian damage and preserves ovarian reserve following ovarian torsion.
本研究旨在探讨依诺肝素（E）对体外受精大鼠卵巢缺血（I）和缺血再灌注（I/R）损伤的保护作用。
共 56 只成年雌性 Sprague-Dawley 白化大鼠随机分为 6 组，每组 8 只：假手术组、缺血组、I/R 组、Sham+E 组、I+E 组和 I/R+E 组。缺血组双侧附件扭转 3 小时。相比之下，I/R 和 I/R+E 组在随后的 3 小时内进行再灌注。在缺血（I+富含血小板的血浆）或再灌注（I/R+I+富含血小板的血浆）前 30 分钟，给予依诺肝素（0.5mg/kg 皮下注射）。所有组均在手术前后通过腹腔内注射 150-300 国际单位 IU/kg 孕马血清促性腺激素刺激卵巢。
当评估中期 II 卵母细胞数量时，I 组和 I+E 组（p=0.001）以及 I/R 组和 I/R+E 组（p=0.000）之间存在显著差异。当比较 I 和 I+E 组以及 I/R 和 I/R+E 组时，发现应用 E 可增加受精卵母细胞的数量。I/R+E 组的第 2 天胚胎数量高于 I/R 组。I/R 组和 I/R+E 组之间，I/R+E 组的 1 级胚胎数量较高，差异有统计学意义（p=0.003）。在组内比较抗苗勒管激素值时，I 组和 I/R 组的下降幅度最大。
依诺肝素可有效减轻卵巢扭转后卵巢损伤，保护卵巢储备。
