Faculty of Chemistry and Chemical Engineering, University of Maribor, 2000 Maribor, Slovenia.
FunGlass-Center for Functional and Surface Functionalized Glass, Alexander Dubček University of Trenčín, 91150 Trenčín, Slovakia.
Biosensors (Basel). 2022 Mar 4;12(3):160. doi: 10.3390/bios12030160.
Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigate suitability of HepG2 cells for biosensor use, different methods of adhesion on stainless steel surfaces were investigated, with three groups of experiments performed in vitro. Cytotoxicity assays, which include the resazurin assay, the neutral red assay (NR), and the Coomassie Brilliant Blue (CBB) assay, were used to determine the viability of HepG2 cells exposed to various concentrations of aflatoxin B1 (AFB1) and isoniazid (INH) in parallel. The viability of the HepG2 cells on the stainless steel surface was quantitatively and qualitatively examined with different microscopy techniques. A simple cell-based electrochemical biosensor was developed by evaluating the viability of the HepG2 cells on the stainless steel surface when exposed to various concentrations of AFB1 and INH by using electrochemical impedance spectroscopy (EIS). The results showed that HepG2 cells can adhere to the metal surface and could be used as part of the biosensor to determine simple hepatotoxic samples.
人类经常接触环境性肝毒素，这可能导致肝衰竭。生物传感器因其高灵敏度和特异性、方便、省时、低成本和极低的检测限，可能是检测肝毒素的最佳候选者。为了研究 HepG2 细胞是否适合用作生物传感器，我们研究了在不锈钢表面上不同的粘附方法，在体外进行了三组实验。使用细胞毒性测定法（包括 Resazurin 测定法、中性红测定法（NR）和考马斯亮蓝测定法（CBB））来平行测定 HepG2 细胞暴露于不同浓度黄曲霉毒素 B1（AFB1）和异烟肼（INH）时的活力。使用不同的显微镜技术对不锈钢表面上 HepG2 细胞的活力进行了定量和定性检测。通过使用电化学阻抗谱（EIS）评估 HepG2 细胞在暴露于不同浓度 AFB1 和 INH 时在不锈钢表面上的活力，开发了一种简单的基于细胞的电化学生物传感器。结果表明，HepG2 细胞可以附着在金属表面上，并可作为生物传感器的一部分，用于检测简单的肝毒性样本。
Zotero 插件
