Xia Daosong, Zheng Renwen, Huang Jianhua, Lu Sihan, Tang Qingfeng
Anhui Province Key Laboratory of Integrated Pest Management on Crops, Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
Insects. 2022 Mar 5;13(3):259. doi: 10.3390/insects13030259.
Odorant-degrading enzymes (ODEs) play an important role in rapidly degrading and inactivating odorant molecules that have completed information transmission, as well as in maintaining the stability and sensitivity of insect olfactory sensing systems. Glutathione S-transferases (GSTs), as a group of ODEs, supposedly bear the ability to catalyze the conjugation of glutathione (GSH) and xenobiotic odorant molecules in the degrading process. However, there are few reports regarding the role of the GST genes of in the degrading process. Thus, we characterized 13 full-length genes encoding GST sequences from , of which only contained a high abundance in the antennae. Ligand-binding assays implied that SzeaGSTd1 was able to catalyze the conjugation of GSH with 2, 4-dinitrochlorobenzene (CDNB). We investigated whether recombinant SzeaGSTd1 bears the ability to degrade the volatile molecules of the host; among the host volatiles, and found capryl alcohol to be a suitable substrate for SzeaGSTd1. These results strongly suggest that SzeaGSTd1 probably plays a role in auxiliary host location by degrading the host volatiles of capryl alcohol and exhibits a potential biological function in the olfactory sensing system of . Knowledge of the potential functions of SzeaGSTd1 will provide new ideas for biological control strategies for .
气味降解酶（ODEs）在快速降解和失活已完成信息传递的气味分子以及维持昆虫嗅觉传感系统的稳定性和敏感性方面发挥着重要作用。谷胱甘肽S-转移酶（GSTs）作为一类ODEs，据推测在降解过程中具有催化谷胱甘肽（GSH）与外源气味分子结合的能力。然而，关于GST基因在降解过程中的作用的报道很少。因此，我们对斜纹夜蛾中13个编码GST序列的全长基因进行了表征，其中只有一个在触角中高丰度表达。配体结合试验表明，斜纹夜蛾GSTd1能够催化GSH与2,4-二硝基氯苯（CDNB）的结合。我们研究了重组斜纹夜蛾GSTd1是否具有降解寄主挥发性分子的能力；在寄主挥发性物质中，发现辛醇是斜纹夜蛾GSTd1的合适底物。这些结果强烈表明，斜纹夜蛾GSTd1可能通过降解寄主挥发性物质辛醇在辅助寄主定位中发挥作用，并在斜纹夜蛾的嗅觉传感系统中展现出潜在的生物学功能。对斜纹夜蛾GSTd1潜在功能的了解将为斜纹夜蛾的生物防治策略提供新的思路。
