Jiang Xinyi, Shen Jiacheng, Lin Peng, Hou Youming
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Insects. 2025 Jan 5;16(1):43. doi: 10.3390/insects16010043.
Insects have highly developed olfactory systems in which cytochrome P450s (CYPs) were involved as odor-degrading enzymes throughout the olfactory recognition of odor compounds by insects to avoid continuous stimulation of signaling molecules and thus damage to the olfactory nervous. To understand whether the highly expressed CYPs in the antennae play an olfactory function in worker, in this study, we find six highly expressed antennal CYPs from the transcriptome of . Multiple sequence alignment and phylogenetic analysis divided them into two families: the CYP3 family (, ) and the CYP4 family (, , , ). The expression patterns of these six CYPs were analyzed by RT-qPCR, which revealed that and were only highly expressed in the antennae of adult workers. The expression of and in workers was markedly diminished after feeding with dsRNA. The electroantennography (EAG) assay demonstrated that the silencing of either or resulted in a notable reduction in the EAG response of workers to 2-ethyl-3,6(5)-dimethylpyrazine (EDMP). Furthermore, the trajectory behavior assay showed that the worker's range and speed of movement in response to EDMP significant decreased after the silencing of and . The findings indicated that both and were implicated in the recognition of EDMP by .
昆虫具有高度发达的嗅觉系统，其中细胞色素P450（CYPs）作为气味降解酶参与昆虫对气味化合物的整个嗅觉识别过程，以避免信号分子的持续刺激，从而保护嗅觉神经免受损伤。为了了解触角中高表达的CYPs是否在工蜂中发挥嗅觉功能，在本研究中，我们从……的转录组中发现了6个高表达的触角CYPs。多序列比对和系统发育分析将它们分为两个家族：CYP3家族（……）和CYP4家族（……）。通过RT-qPCR分析了这6个CYPs的表达模式，结果显示……仅在成年工蜂的触角中高表达。用dsRNA喂食后，工蜂中……的表达显著降低。触角电位（EAG）测定表明，沉默……或……会导致工蜂对2-乙基-3,6(5)-二甲基吡嗪（EDMP）的EAG反应显著降低。此外，轨迹行为测定表明，沉默……和……后，工蜂对EDMP的反应范围和移动速度显著下降。研究结果表明，……和……都参与了工蜂对EDMP的识别。
