Wu Hailong, Yue Shuaili, Huang Yong, Zhao Xinping, Cao Haiqun, Liao Min
Anhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Insects. 2022 Mar 14;13(3):283. doi: 10.3390/insects13030283.
Long noncoding RNAs (lncRNAs) are important regulatory factors in multiple biological processes, and several lncRNAs are known to respond to insecticides. However, the lncRNA functions that are associated with terpinen-4-ol resistance in the red flour beetle () have not yet been identified. In this study, we determined the differentially transcribed lncRNAs between fumigated and control experimental groups. In the six libraries that underwent RNA sequencing, 34,546 transcripts were identified, including 8267 novel lncRNAs, 4155 novel mRNAs, 1151 known lncRNAs, and 20,973 known mRNAs. Among these, we found that the expression of 1858 mRNAs and 1663 lncRNAs was significantly different in the fumigated group compared with the control group. Among the differentially transcribed lncRNAs, 453 were up-regulated and 1210 were down-regulated lncRNAs. In addition, we identified the regulatory function targets of the lncRNAs. Functionally, all lncRNAs and target genes associated with terpinen-4-ol metabolism were enriched in several metabolic pathways, like the ATP-binding cassette transporter, pentose interconversion, and glucuronate interconversion. To the best of our knowledge, this study represents the first global identification of lncRNAs and their potential association with terpinen-4-ol metabolism in the red flour beetle. These results will provide reference information for studies on the resistance to terpinen-4-ol and other essential oil compounds and chemical pesticides, as well as an understanding of other biological processes in .
长链非编码RNA（lncRNAs）是多种生物过程中的重要调控因子，已知有几种lncRNAs对杀虫剂有反应。然而，与赤拟谷盗对萜品醇4-醇抗性相关的lncRNA功能尚未确定。在本研究中，我们确定了熏蒸实验组和对照组之间差异转录的lncRNAs。在进行RNA测序的六个文库中，共鉴定出34,546个转录本，包括8267个新的lncRNAs、4155个新的mRNAs、1151个已知的lncRNAs和20,973个已知的mRNAs。其中，我们发现熏蒸组与对照组相比，1858个mRNAs和1663个lncRNAs的表达存在显著差异。在差异转录的lncRNAs中，453个上调，1210个下调。此外，我们还确定了lncRNAs的调控功能靶点。在功能上，所有与萜品醇4-醇代谢相关的lncRNAs和靶基因都富集在几个代谢途径中，如ATP结合盒转运蛋白、戊糖互变和葡糖醛酸互变。据我们所知，本研究首次全面鉴定了赤拟谷盗中的lncRNAs及其与萜品醇4-醇代谢的潜在关联。这些结果将为研究赤拟谷盗对萜品醇4-醇和其他精油化合物及化学农药的抗性以及了解其其他生物过程提供参考信息。
