基于 H-NMR 和 LC-MS 的施粉煤灰处理酸性矿山排水灌溉马铃薯品种的代谢组学分析。
H-NMR and LC-MS Based Metabolomics Analysis of Potato ( L.) Cultivars Irrigated with Fly Ash Treated Acid Mine Drainage.
机构信息
Department of Agriculture and Animal Health, College of Agriculture and Environmental Sciences, Private Bag X6, Florida 1710, South Africa.
Arkansas Children's Nutrition Center, 15 Children's Way, Little Rock, AR 72202, USA.
出版信息
Molecules. 2022 Feb 10;27(4):1187. doi: 10.3390/molecules27041187.
H NMR and LC-MS, commonly used metabolomics analytical platforms, were used to annotate the metabolites found in potato ( L.) irrigated with four different treatments based on FA to AMD ratios, namely: control (0% AMD; tap water), 1:1 (50% AMD), 3:1 (75% AMD is 75% FA: AMD), and 100% AMD (untreated). The effects of stress on plants were illustrated by the primary metabolite shifts in the region from δ 0.0 to δ 4.0 and secondary metabolites peaks were prominent in the region ranging from δ 4.5 to δ 8.0. The 1:3 irrigation treatment enabled, in two potato cultivars, the production of significantly high concentrations of secondary metabolites due to the 75% FA: AMD content in the irrigation mixture, which induced stress. The findings suggested that 1:1 irrigation treatment induced production of lower amounts of secondary metabolites in all crops compared to crops irrigated with untreated acid mine drainage treatment and with other FA-treated AMD solutions.
使用 NMR 和 LC-MS 这两种常用的代谢组学分析平台，根据 FA 与 AMD 的比例，对用四种不同处理方式灌溉的马铃薯（ L.）中发现的代谢物进行注释，这四种处理方式分别是：对照（0%AMD；自来水）、1:1（50%AMD）、3:1（75%AMD 是 75%FA：AMD）和 100%AMD（未处理）。胁迫对植物的影响表现在 δ 0.0 到 δ 4.0 区域的主要代谢物变化上，而 δ 4.5 到 δ 8.0 区域的次生代谢物峰则较为明显。在两种马铃薯品种中，1:3 的灌溉处理由于灌溉混合物中含有 75%FA：AMD，导致产生了显著的高浓度次生代谢物，从而产生了胁迫。研究结果表明，与用未处理的酸性矿山排水处理和其他 FA 处理的 AMD 溶液灌溉的作物相比，1:1 灌溉处理在所有作物中诱导产生的次生代谢物数量较少。
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